Construct: ORF TRCN0000468561
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012927.1_s317c1
- Derived from:
- ccsbBroadEn_02806
- DNA Barcode:
- TCCACCCTGCGGGAGTGCATTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPN3 (23596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468561
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23596 | OPN3 | opsin 3 | NM_014322.3 | 100% | 100% | |
| 2 | mouse | 13603 | Opn3 | opsin 3 | NM_010098.3 | 85% | 87% | (many diffs) |
| 3 | mouse | 13603 | Opn3 | opsin 3 | XM_006496642.2 | 59.7% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1272
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ctcggggaac cgcagcggcg gccacggcta ctgggacggc ggcggggccg 121 cgggcgctga ggggccggcg ccggcgggga cactgagccc cgcgcccctc ttcagccccg 181 gcacctacga gcgcctggcg ctgctgctgg gctccattgg gctgctgggc gtcggcaaca 241 acctgctggt gctcgtcctc tactacaagt tccagcggct ccgcactccc actcacctcc 301 tcctggtcaa catcagcctc agcgacctgc tggtgtccct cttcggggtc acctttacct 361 tcgtgtcctg cctgaggaac ggctgggtgt gggacaccgt gggctgcgtg tgggacgggt 421 ttagcggcag cctcttcggg attgtttcca ttgccaccct aaccgtgctg gcctatgaac 481 gttacattcg cgtggtccat gccagagtga tcaatttttc ctgggcctgg agggccatta 541 cctacatctg gctctactca ctggcgtggg caggagcacc tctcctggga tggaacaggt 601 acatcctgga cgtacacgga ctaggctgca ctgtggactg gaaatccaag gatgccaacg 661 attcctcctt tgtgcttttc ttatttcttg gctgcctggt ggtgcccctg ggtgtcatag 721 cccattgcta tggccatatt ctatattcca ttcgaatgct tcgttgtgtg gaagatcttc 781 agacaattca agtgatcaag attttaaaat atgaaaagaa actggccaaa atgtgctttt 841 taatgatatt caccttcctg gtctgttgga tgccttatat cgtgatcTGC TTCTTGGTGG 901 TTAATGGTCA TGGTCACCTG GTCACTCCAA CAATATCTAT TGTTTCGTAC CTCTTTGCTA 961 AATCGAACAC TGTATACAAT CCAGTGATTT ATGTCTTCAT GATCAGAAAG TTTCGAAGAT 1021 CCCTTTTGCA GCTTCTGTGC CTCCGACTGC TGAGGTGCCA GAGGCCTGCT AAAGACCTAC 1081 CAGCAGCTGG AAGTGAAATG CAGATCAGAC CCATTGTGAT GTCACAGAAA GATGGGGACA 1141 GGCCAAAGAA AAAAGTGACT TTCAACTCTT CTTCCATCAT TTTTATCATC ACCAGTGATG 1201 AATCACTGTC AGTTGACGAC AGCGACAAAA CCAATGGGTC CAAAGTTGAT GTAATCCAAG 1261 TTCGTCCTTT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCC ACCCTGCGGG AGTGCATTAC 1441 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t