Construct: ORF TRCN0000468565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000587.1_s317c1
- Derived from:
- ccsbBroadEn_07540
- DNA Barcode:
- TTCCTGCAACTGCAATAATGCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAE1 (10055)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468565
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10055 | SAE1 | SUMO1 activating enzyme sub... | NM_005500.3 | 99.9% | 99.7% | 1031G>A |
| 2 | human | 10055 | SAE1 | SUMO1 activating enzyme sub... | NM_001145714.2 | 76.8% | 71.3% | 731_732ins145;798_799ins95 |
| 3 | human | 10055 | SAE1 | SUMO1 activating enzyme sub... | NM_001145713.2 | 73.9% | 65.1% | 730_731ins215;816G>A;824_897del |
| 4 | human | 341056 | LOC341056 | SUMO1 activating enzyme sub... | NR_027288.1 | 46.7% | (many diffs) | |
| 5 | human | 10055 | SAE1 | SUMO1 activating enzyme sub... | NR_027280.2 | 39.7% | (many diffs) | |
| 6 | mouse | 56459 | Sae1 | SUMO1 activating enzyme sub... | NM_001285891.1 | 86.3% | 92% | (many diffs) |
| 7 | mouse | 56459 | Sae1 | SUMO1 activating enzyme sub... | NM_019748.3 | 86.3% | 92% | (many diffs) |
| 8 | mouse | 56459 | Sae1 | SUMO1 activating enzyme sub... | NM_001285892.1 | 79.2% | 84.3% | (many diffs) |
| 9 | mouse | 56459 | Sae1 | SUMO1 activating enzyme sub... | XM_011250653.1 | 66.9% | 66.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1104
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggagaaggag gaggctggcg gcggcattag cgaggaggag gcggcacagt 121 atgaccggca gatccgcctg tggggactgg aggcccagaa acggctgcgg gcctctcggg 181 tgcttcttgt cggcttgaaa ggacttgggg ctgaaattgc caagaatctc atcttggcag 241 gagtgaaagg actgaccatg ctggatcacg aacaggtaac tccagaagat cccggagctc 301 agttcttgat tcgtactggg tctgttggcc gaaatagggc tgaagcctct ttggagcgag 361 ctcagaatct caaccccatg gtggatgtga aggtggacac tgaggatata gagaagaaac 421 cagagtcatt tttcactcaa ttcgatgctg tgtgtctgac ttgctgctcc agggatgtca 481 tagttaaagt tgaccagatc tgtcacaaaa atagcatcaa gttctttaca ggagatgttt 541 ttggctacca tggatacaca tttgccaatc taggagagca tgagtttgta gaggagaaaa 601 ctaaagttgc caaagttagc caaggagtag aagatgggcc cgacaccaag agagcaaaac 661 ttgattcttc tgagacaacg atggtcaaaa agaaggtggT CTTCTGCCCT GTTAAAGAAG 721 CCCTGGAGGT GGACTGGAGC AGTGAGAAAG CAAAGGCTGC TCTGAAGCGC ACGACCTCCG 781 ACTACTTTCT CCTTCAAGTG CTCTTAAAGT TCCGTACAGA TAAAGGAAGA GATCCCAGTT 841 CTGATACATA TGAGGAAGAT TCTGAGTTGT TGCTCCAGAT ACGAAATGAT GTGCTTGACT 901 CACTGGGTAT TAGTCCTGAC CTGCTTCCTG AGGACTTTGT CAGGTACTGC TTCTCCGAGA 961 TGGCCCCAGT GTGTGCGGTG GTTGGAGGGA TTTTGGCACA GGAAATTGTG AAGGCCCTGT 1021 CTCAGCGGGA CCCTCCTCAC AACAACTTCT TCTTCTTCGA TGGCATGAAG GGGAATGGGA 1081 TTGTGGAGTG CCTTGACCCC AAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TCCTGCAACT 1261 GCAATAATGC GTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att