Transcript: Mouse NM_001285891.1

Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Sae1 (56459)
Length:
1850
CDS:
147..1199

Additional Resources:

NCBI RefSeq record:
NM_001285891.1
NBCI Gene record:
Sae1 (56459)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001285891.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000040500 GCTGAAATTCCGCACAGATAA pLKO.1 896 CDS 100% 13.200 18.480 N Sae1 n/a
2 TRCN0000301271 GCTGAAATTCCGCACAGATAA pLKO_005 896 CDS 100% 13.200 18.480 N Sae1 n/a
3 TRCN0000040499 CGAATCTTGGAGAGCATGAAT pLKO.1 658 CDS 100% 5.625 7.875 N Sae1 n/a
4 TRCN0000301191 CGAATCTTGGAGAGCATGAAT pLKO_005 658 CDS 100% 5.625 7.875 N Sae1 n/a
5 TRCN0000304251 AGATACGGAATGACGTGTTTG pLKO_005 970 CDS 100% 10.800 7.560 N Sae1 n/a
6 TRCN0000040501 GAGTCCTTCTTCACGAAGTTT pLKO.1 516 CDS 100% 5.625 3.938 N Sae1 n/a
7 TRCN0000301269 GAGTCCTTCTTCACGAAGTTT pLKO_005 516 CDS 100% 5.625 3.938 N Sae1 n/a
8 TRCN0000040502 CCCTCCTCACAACAACTTCTT pLKO.1 1124 CDS 100% 4.950 3.465 N Sae1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001285891.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07540 pDONR223 100% 86.3% 92% None (many diffs) n/a
2 ccsbBroad304_07540 pLX_304 0% 86.3% 92% V5 (many diffs) n/a
3 TRCN0000468565 TTCCTGCAACTGCAATAATGCGTT pLX_317 39.2% 86.3% 92% V5 (many diffs) n/a
Download CSV