Construct: ORF TRCN0000468575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007683.1_s317c1
- Derived from:
- ccsbBroadEn_03892
- DNA Barcode:
- GCGTCTTTTTCTTATGCCGTTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NYX (60506)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468575
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60506 | NYX | nyctalopin | NM_022567.2 | 100% | 100% | |
| 2 | human | 60506 | NYX | nyctalopin | XM_017029709.1 | 100% | 100% | |
| 3 | mouse | 236690 | Nyx | nyctalopin | NM_173415.4 | 83.8% | 83.7% | (many diffs) |
| 4 | mouse | 236690 | Nyx | nyctalopin | XM_006527612.3 | 83.8% | 83.7% | (many diffs) |
| 5 | mouse | 236690 | Nyx | nyctalopin | XM_006527613.3 | 83.8% | 83.7% | (many diffs) |
| 6 | mouse | 236690 | Nyx | nyctalopin | XM_006527614.3 | 83.8% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1512
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaggccga gggatgttgg tcctgcttct gcatgcggtg gtcctcggcc 121 tgcccagcgc ctgggccgtg ggggcctgcg cccgcgcttg tcccgccgcc tgcgcctgca 181 gcaccgtgga gcgcggctgc tcggtgcgct gcgaccgcgc gggcctcctg cgggtgccgg 241 ccgagctccc gtgcgaggcg gtctccatcg acctggaccg gaacggcctg cgcttcctgg 301 gcgagcgagc cttcggcacg ctgccgtcct tgcgccgcct gtcgctgcgc cacaacaacc 361 tgtccttcat cacgcccggc gccttcaagg gcctgccgcg cctggctgag ctgcgcctgg 421 cgcacaacgg cgacctgcgc tacctgcacg cgcgcacctt cgcggcgctc agccgcctgc 481 gccgcctaga cctagcagcc tgccgcctct tcagcgtgcc cgagcgcctc ctggccgaac 541 tgccggccct gcgcgaactc gccgccttcg acaacctgtt ccgccgcgtg ccgggcgcgc 601 tgcgcggcct ggccaacctg acgcacgcgc acctggagcg cggccgcatc gaggcggtgg 661 cctccagctc gctgcagggc ctgcgccgcc tgcgctcgct cagcctgcag gccaaccgcg 721 tccgtgccgt gcacgctggc gccttcgggg actgtggcgt cctggagcat ctgctgctca 781 acgacaacct gctggccgag ctcccggccg acgccttccg cggcctgcgg cgcctgcgca 841 cgctcaacct gggtggcaac gcgctggacc gcgtggcgcg cgcctggttc gctgacctgg 901 ccgagctcga gctgctctac ctggaccgca acagcatcgc cttcgtggag gagggcgcct 961 tccagaacct ctcgggtctc ctcgcgctgc acctcaacgg caaccgcctc accgtgctcg 1021 cctgggtcgc cttccagccc ggcttcttcc tgggccgcct cttcctcttc cgcaacccgt 1081 ggtgctgcga ctgccgtctg gagtggctga gggactggat ggagggctcc ggacgtgtca 1141 ccgacgtgcc gtgcgcctcc ccgggctccg tggccggcct ggacctcagc caggtgacct 1201 tcgggcgctc cTCCGATGGC CTCTGTGTGG ACCCCGAGGA GCTGAACCTC ACCACGTCCA 1261 GTCCAGGCCC GTCCCCAGAA CCAGCGGCCA CCACCGTGAG CAGGTTCAGC AGCCTCCTCT 1321 CCAAGCTGCT GGCCCCGAGG GTCCCGGTGG AGGAGGCGGC CAACACCACT GGGGGGCTGG 1381 CCAACGCCTC CCTGTCCGAC AGCCTCTCCT CCCGTGGGGT GGGAGGCGCG GGCCGGCAGC 1441 CCTGGTTTCT CCTCGCCTCT TGTCTCCTGC CCAGCGTGGC CCAGCACGTG GTGTTTGGCC 1501 TGCAGATGGA CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCG TCTTTTTCTT ATGCCGTTGC 1681 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t