Construct: ORF TRCN0000468578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004126.1_s317c1
- Derived from:
- ccsbBroadEn_08380
- DNA Barcode:
- AGTACGTTCTATCTCATTAAGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPIN2A (54466)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54466 | SPIN2A | spindlin family member 2A | NM_019003.4 | 99.6% | 99.6% | 118C>A;132G>A;136C>T |
| 2 | human | 54466 | SPIN2A | spindlin family member 2A | XM_005262016.4 | 99.6% | 99.6% | 118C>A;132G>A;136C>T |
| 3 | human | 54466 | SPIN2A | spindlin family member 2A | XM_005262017.4 | 99.6% | 99.6% | 118C>A;132G>A;136C>T |
| 4 | human | 54466 | SPIN2A | spindlin family member 2A | XM_005262019.3 | 99.6% | 99.6% | 118C>A;132G>A;136C>T |
| 5 | human | 54466 | SPIN2A | spindlin family member 2A | XM_017029598.1 | 99.6% | 99.6% | 118C>A;132G>A;136C>T |
| 6 | human | 474343 | SPIN2B | spindlin family member 2B | NM_001006681.1 | 99.3% | 99.2% | (many diffs) |
| 7 | human | 474343 | SPIN2B | spindlin family member 2B | NM_001006682.2 | 99.3% | 99.2% | (many diffs) |
| 8 | human | 474343 | SPIN2B | spindlin family member 2B | NM_001006683.1 | 99.3% | 99.2% | (many diffs) |
| 9 | human | 474343 | SPIN2B | spindlin family member 2B | XM_005262009.5 | 99.3% | 99.2% | (many diffs) |
| 10 | human | 474343 | SPIN2B | spindlin family member 2B | XM_005262010.2 | 99.3% | 99.2% | (many diffs) |
| 11 | human | 474343 | SPIN2B | spindlin family member 2B | XM_011530788.3 | 99.3% | 99.2% | (many diffs) |
| 12 | human | 474343 | SPIN2B | spindlin family member 2B | XM_017029552.2 | 99.3% | 99.2% | (many diffs) |
| 13 | human | 474343 | SPIN2B | spindlin family member 2B | XM_005262008.5 | 84.3% | 84.2% | (many diffs) |
| 14 | human | 54466 | SPIN2A | spindlin family member 2A | XM_005262018.5 | 60.4% | 60.4% | (many diffs) |
| 15 | human | 474343 | SPIN2B | spindlin family member 2B | NM_001282461.2 | 60.3% | 60% | (many diffs) |
| 16 | human | 474343 | SPIN2B | spindlin family member 2B | NM_001282462.2 | 60.3% | 60% | (many diffs) |
| 17 | human | 474343 | SPIN2B | spindlin family member 2B | XM_011530789.2 | 60.3% | 60% | (many diffs) |
| 18 | mouse | 278240 | Spin2c | spindlin family, member 2C | NM_001005370.1 | 82.7% | 81% | (many diffs) |
| 19 | mouse | 278240 | Spin2c | spindlin family, member 2C | XM_006528868.3 | 82.7% | 81% | (many diffs) |
| 20 | mouse | 278240 | Spin2c | spindlin family, member 2C | XM_006528869.3 | 82.7% | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gacccccaac gcacaggaag ccgaagggca acaaaccagg gcagctgcag 121 gacgggccac tgggtctgca aacatgacaa agaaaaaagt ctcccaaaag aagcagagag 181 gcagaccttc atcccaaccc tgcaggaaca tcgtgggctg cagaatttct catggatgga 241 aggaaggaga tgagcccatc acgcagtgga aaggaaccgt tctggatcag gtgcctataa 301 atccctctct ttatctggtg aaatatgatg gaattgactg tgtctatgga ctggaacttc 361 acagagatga aagggttttg tctcttaaaa ttctttctga cagggtggca tcatctcaca 421 ttagtgatgc caaccttgca aataccataa ttGGCAAAGC AGTGGAACAC ATGTTTGAGG 481 GAGAGCATGG TTCTAAGGAT GAATGGAGGG GGATGGTCTT AGCTCAAGCA CCTATCATGA 541 AAGCCTGGTT TTATATTACC TATGAGAAAG ATCCTGTCTT GTACATGTAC CAGCTTCTAG 601 ATGATTATAA GGAAGGTGAC CTCCGCATCA TGCCAGAATC CAGTGAGTCT CCTCCAACAG 661 AGAGGGAGCC AGGAGGAGTT GTAGATGGCC TAATAGGTAA GCATGTGGAA TATACCAAAG 721 AAGATGGCTC CAAAAGGATC GGCATGGTCA TTCACCAAGT GGAAACCAAA CCCTCTGTGT 781 ATTTCATCAA GTTTGATGAT GATTTCCATA TCTATGTCTA CGATTTGGTG AAAAAGTCCT 841 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAAGTAC GTTCTATCTC ATTAAGTTAA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt