Construct: ORF TRCN0000468595
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007858.1_s317c1
- Derived from:
- ccsbBroadEn_10804
- DNA Barcode:
- AGGACCGTGTTACCCAGCCCCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB2 (2048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468595
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2048 | EPHB2 | EPH receptor B2 | NM_001309192.2 | 51.5% | 51.2% | (many diffs) |
| 2 | human | 2048 | EPHB2 | EPH receptor B2 | XM_006710442.4 | 50.4% | 49.7% | (many diffs) |
| 3 | human | 2048 | EPHB2 | EPH receptor B2 | NM_017449.4 | 48.5% | 48.2% | (many diffs) |
| 4 | human | 2048 | EPHB2 | EPH receptor B2 | NM_004442.7 | 48.4% | 48.2% | (many diffs) |
| 5 | human | 2048 | EPHB2 | EPH receptor B2 | XM_006710441.4 | 47.4% | 46% | (many diffs) |
| 6 | human | 2048 | EPHB2 | EPH receptor B2 | NM_001309193.2 | 45.3% | 45.1% | (many diffs) |
| 7 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538533.3 | 45.6% | 49.4% | (many diffs) |
| 8 | mouse | 13844 | Ephb2 | Eph receptor B2 | NM_001290753.2 | 44.1% | 47.9% | (many diffs) |
| 9 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538534.2 | 44% | 47.8% | (many diffs) |
| 10 | mouse | 13844 | Ephb2 | Eph receptor B2 | NM_010142.4 | 44% | 47.9% | (many diffs) |
| 11 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538531.3 | 44% | 47.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctgcggagg ctgggggccg cgctgctgct gctgccgctg ctcgccgccg 121 tggaagaaac gctaatggac tccactacag cgactgctga gctgggctgg atggtgcatc 181 ctccatcagg gtgggaagag gtgagtggct acgatgagaa catgaacacg atccgcacgt 241 accaggtgtg caacgtgttt gagtcaagcc agaacaactg gctacggacc aagtttatcc 301 ggcgccgtgg cgcccaccgc atccacgtgg agatgaagtt ttcggtgcgt gactgcagca 361 gcatccccag cgtgcctggc tcctgcaagg agaccttcaa cctctattac tatgaggctg 421 actttgactc ggccaccaag accttcccca actggatgga gaatccatgg gtgaaggtgg 481 ataccattgc agccgacgag agcttctccc aggtggacct gggtggccgc gtcatgaaaa 541 tcaacaccga ggtgcggagc ttcggacctg tgtcccgcag cggcttctac ctggccttcc 601 aggactatgg cggctgcatg tccctcatcg ccgtgcgtgt cttctaccgc aagtgccccc 661 gcatcatcca gaatggcgcc atcttccagg aaaccctgtc gggggctgag agcacatcgc 721 tggtggctgc ccggggcagc tgcatcgcca atgcggaaga ggtggatgta cccatcaagc 781 tctactgtaa cggggacggc gagtggctgg tgcccatcgg gcgctgcatg tgcaaagcag 841 gcttcgaggc cgttgagaat ggcaccgtct gccgaggttg tccatctggg actttcaagg 901 ccaaccaagg ggatgaggcc tgtacccact gtcccatcaa cagccggacc acttctgaag 961 gggccaccaa ctgtgtctgc cgcaatggct actacagagc agacctggac cccctggaca 1021 tgccctgcac aaccatcccc tccgcgcccc aggctgtgat ttccagtgtc aatgagacct 1081 ccctcatgct ggagtggacc cctccccgcg actccggagg ccgagaggac ctcgtctaca 1141 acaTCATCTG CAAGAGCTGT GGCTCGGGCC GGGGTGCCTG CACCCGCTGC GGGGACAATG 1201 TACAGTACGC ACCACGCCAG CTAGGCCTGA CCGAGCCACG CATTTACATC AGTGACCTGC 1261 TGGCCCACAC CCAGTACACC TTCGAGATCC AGGCTGTGAA CGGCGTTACT GACCAGAGCC 1321 CCTTCTCGCC TCAGTTCGCC TCTGTGAACA TCACCACCAA CCAGGCAGCT CCATCGGCAG 1381 TGTCCATCAT GCATCAGGTG AGCCGCACCG TGGACAGCAT TACCCTGTCG TGGTCCCAGC 1441 CGGACCAGCC CAATGGCGTG ATCCTGGACT ATGAGCTGCA GTACTATGAG AAGATGAAGA 1501 CACAGAGAAG TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGG ACCGTGTTAC CCAGCCCCAG 1681 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t