Construct: ORF TRCN0000468601
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017885.1_s317c1
- Derived from:
- ccsbBroadEn_09842
- DNA Barcode:
- TGCGTCCATACAGTAGTGACATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZCCHC24 (219654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468601
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219654 | ZCCHC24 | zinc finger CCHC-type conta... | NM_153367.4 | 99.8% | 99.5% | 128A>T |
| 2 | human | 219654 | ZCCHC24 | zinc finger CCHC-type conta... | XM_011539452.3 | 67.8% | 67.6% | (many diffs) |
| 3 | mouse | 71918 | Zcchc24 | zinc finger, CCHC domain co... | NM_001101433.1 | 91.9% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cctgctgtcg gccatcgaca cgagcgccgc ctcggtgtac cagcccgccc 121 agctgctcaa ctgggtctac ctgtcgctgc aggacacgca ccaggctagc gccttcgatg 181 ccttccggcc cgtgccgacc gccggcgccg cacccccgga gctggccttc ggcaagggcc 241 gccccgagca gctgggctcg cccctgcact ccagctatct caacagcttc ttccagctgc 301 agcgcggaga ggcgctgagc aacagtgtgt acaagggcgc ctcaccctat ggctccctca 361 acaacatcgc cgatggccTC AGCTCCCTCA CCGAGCACTT CTCAGACCTG ACCCTCACCT 421 CCGAGGCTCG CAAGCCCAGC AAGCGGCCCC CACCCAACTA CCTGTGCCAC CTGTGCTTCA 481 ACAAAGGACA CTACATCAAG GACTGCCCCC AGGCACGCCC CAAAGGCGAG GGCCTGACTC 541 CATACCAGGG CAAAAAGCGC TGCTTCGGCG AGTACAAGTG TCCCAAGTGC AAGAGAAAAT 601 GGATGAGCGG GAACTCCTGG GCCAACATGG GGCAGGAGTG CATCAAGTGC CACATCAACG 661 TGTATCCACA CAAGCAGAGA CCCCTGGAGA AGCCCGACGG CCTGGACGTG TCCGACCAGA 721 GCAAGGAGCA CCCGCAGCAC CTCTGCGAGA AGTGCAAGGT CCTGGGCTAC TACTGCCGTC 781 GCGTGCAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGCGTC CATACAGTAG TGACATTCAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt