Construct: ORF TRCN0000468638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010415.1_s317c1
- Derived from:
- ccsbBroadEn_04407
- DNA Barcode:
- GACTATGGAAAGAGTCTCTTCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGAT2 (84649)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468638
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84649 | DGAT2 | diacylglycerol O-acyltransf... | NM_032564.5 | 100% | 100% | |
| 2 | human | 84649 | DGAT2 | diacylglycerol O-acyltransf... | XM_011545304.2 | 91% | 90.4% | (many diffs) |
| 3 | human | 84649 | DGAT2 | diacylglycerol O-acyltransf... | NM_001253891.1 | 88.9% | 88.9% | 119_120ins129 |
| 4 | mouse | 67800 | Dgat2 | diacylglycerol O-acyltransf... | NM_026384.3 | 89.5% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1230
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaccctcata gccgcctact ccggggtcct gcgcggcgag cgtcaggccg 121 aggctgaccg gagccagcgc tctcacggag gacctgcgct gtcgcgcgag gggtctggga 181 gatggggcac tggatccagc atcctctccg ccctccagga cctcttctct gtcacctggc 241 tcaataggtc caaggtggaa aagcagctac aggtcatctc agtgctccag tgggtcctgt 301 ccttccttgt actgggagtg gcctgcagtg ccatcctcat gtacatattc tgcactgatt 361 gctggctcat cgctgtgctc tacttcactt ggctggtgtt tgactggaac acacccaaga 421 aaggtggcag gaggtcacag tgggtccgaa actgggctgt gtggcgctac tttcgagact 481 actttcccat ccagctggtg aagacacaca acctgctgac caccaggaac tatatctttg 541 gataccaccc ccatggtatc atgggcctgg gtgccttctg caacttcagc acagaggcca 601 cagaagtgag caagaagttc ccaggcatac ggccttacct ggctacactg gcaggcaact 661 tccgaatgcc tgtgttgagg gagtacctga tgtctggagg tatctgccct gtcagccggg 721 acaccataga ctatttgctt tcaaagaatg ggagtggcaa tgctatcatc atcgtggTCG 781 GGGGTGCGGC TGAGTCTCTG AGCTCCATGC CTGGCAAGAA TGCAGTCACC CTGCGGAACC 841 GCAAGGGCTT TGTGAAACTG GCCCTGCGTC ATGGAGCTGA CCTGGTTCCC ATCTACTCCT 901 TTGGAGAGAA TGAAGTGTAC AAGCAGGTGA TCTTCGAGGA GGGCTCCTGG GGCCGATGGG 961 TCCAGAAGAA GTTCCAGAAA TACATTGGTT TCGCCCCATG CATCTTCCAT GGTCGAGGCC 1021 TCTTCTCCTC CGACACCTGG GGGCTGGTGC CCTACTCCAA GCCCATCACC ACTGTTGTGG 1081 GAGAGCCCAT CACCATCCCC AAGCTGGAGC ACCCAACCCA GCAAGACATC GACCTGTACC 1141 ACACCATGTA CATGGAGGCC CTGGTGAAGC TCTTCGACAA GCACAAGACC AAGTTCGGCC 1201 TCCCGGAGAC TGAGGTCCTG GAGGTGAACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACTA 1381 TGGAAAGAGT CTCTTCATGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1441 tgaaagatt