Construct: ORF TRCN0000468641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000278.1_s317c1
- Derived from:
- ccsbBroadEn_07572
- DNA Barcode:
- CGCCGGCGATTATGGAGGTGAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RCAN2 (10231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468641
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10231 | RCAN2 | regulator of calcineurin 2 | NM_005822.3 | 87.5% | 87.5% | 591_592ins84 |
| 2 | human | 10231 | RCAN2 | regulator of calcineurin 2 | NM_001251973.1 | 67.9% | 63.4% | (many diffs) |
| 3 | human | 10231 | RCAN2 | regulator of calcineurin 2 | NM_001251974.1 | 67.9% | 63.4% | (many diffs) |
| 4 | human | 10231 | RCAN2 | regulator of calcineurin 2 | XM_011514226.1 | 67.9% | 63.4% | (many diffs) |
| 5 | human | 10231 | RCAN2 | regulator of calcineurin 2 | XM_024446301.1 | 67.9% | 63.4% | (many diffs) |
| 6 | mouse | 53901 | Rcan2 | regulator of calcineurin 2 | NM_001286654.1 | 78.3% | 85.3% | (many diffs) |
| 7 | mouse | 53901 | Rcan2 | regulator of calcineurin 2 | NM_030598.2 | 78.3% | 85.3% | (many diffs) |
| 8 | mouse | 53901 | Rcan2 | regulator of calcineurin 2 | NM_001286653.1 | 60% | 61.6% | (many diffs) |
| 9 | mouse | 53901 | Rcan2 | regulator of calcineurin 2 | NM_207649.1 | 60% | 61.6% | (many diffs) |
| 10 | mouse | 53901 | Rcan2 | regulator of calcineurin 2 | XM_006524622.1 | 60% | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agcccctagc atggactgtg atgtttccac tctggttgcc tgtgtggtgg 121 atgtcgaggt ctttaccaat caggaggtta aggaaaaatt tgagggactg tttcggactt 181 atgatgactg tgtgacgttc cagctattta agagtttcag acgtgtccgt ataaacttca 241 gcaatcctaa aTCTGCAGCC CGAGCTAGGA TAGAGCTTCA TGAAACCCAA TTCAGAGGGA 301 AAAAATTAAA GCTCTACTTT GCACAGGTTC AGACTCCAGA GACAGATGGA GACAAACTGC 361 ACTTGGCTCC ACCCCAGCCT GCCAAACAGT TTCTCATCTC GCCCCCTTCC TCCCCACCTG 421 TTGGCTGGCA GCCCATCAAC GATGCCACGC CAGTCCTCAA CTATGACCTC CTCTATGCTG 481 TGGCCAAACT AGGACCAGGA GAGAAGTATG AGCTCCATGC AGGGACTGAG TCCACCCCAA 541 GTGTCGTCGT GCACGTGTGC GACAGTGACA TAGAGGAAGA AGAGGACCCA AAGACTTCCC 601 CAAAGCCAAA AATCATCCAA ACTCGGCGTC CTGGCCTGCC ACCCTCCGTG TCCAACTGGG 661 CTGCCTGCTC CTTCTCGATA ATAGCCGTCT CCTCTTTATC ATGCTTTTTC CCCCTGTTGT 721 TTGTCAAAAA GAATTGCCTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGCC GGCGATTATG 901 GAGGTGAATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt