Transcript: Mouse NM_001286654.1

Mus musculus regulator of calcineurin 2 (Rcan2), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Rcan2 (53901)
Length:
3107
CDS:
131..724

Additional Resources:

NCBI RefSeq record:
NM_001286654.1
NBCI Gene record:
Rcan2 (53901)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001286654.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000295529 GTTCCGGACCTATGATGAATG pLKO_005 235 CDS 100% 10.800 15.120 N Rcan2 n/a
2 TRCN0000105808 CCGACGGGTTCGAATAAATTT pLKO.1 283 CDS 100% 15.000 10.500 N Rcan2 n/a
3 TRCN0000288090 CCGACGGGTTCGAATAAATTT pLKO_005 283 CDS 100% 15.000 10.500 N Rcan2 n/a
4 TRCN0000295473 CAATCAACTCCTACCCATTAA pLKO_005 895 3UTR 100% 13.200 9.240 N Rcan2 n/a
5 TRCN0000105809 GCCACACCAGTCCTCAACTAT pLKO.1 509 CDS 100% 5.625 3.938 N Rcan2 n/a
6 TRCN0000288168 GCCACACCAGTCCTCAACTAT pLKO_005 509 CDS 100% 5.625 3.938 N Rcan2 n/a
7 TRCN0000105805 CCTGATACTATTACATGCTAT pLKO.1 1693 3UTR 100% 4.950 3.465 N Rcan2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001286654.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07572 pDONR223 100% 78.3% 85.3% None (many diffs) n/a
2 ccsbBroad304_07572 pLX_304 0% 78.3% 85.3% V5 (many diffs) n/a
3 TRCN0000468641 CGCCGGCGATTATGGAGGTGAATC pLX_317 71.9% 78.3% 85.3% V5 (many diffs) n/a
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