Construct: ORF TRCN0000468654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006969.1_s317c1
- Derived from:
- ccsbBroadEn_04629
- DNA Barcode:
- ACCCTGAACCAGGATCTACTAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRAP2 (112609)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | NM_001346542.2 | 100% | 100% | |
| 2 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | NM_001346544.1 | 100% | 100% | |
| 3 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | NM_138409.4 | 100% | 100% | |
| 4 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | XM_017010220.1 | 100% | 100% | |
| 5 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | NM_001346543.2 | 68.9% | 65.5% | (many diffs) |
| 6 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | NM_001346541.2 | 58% | 58% | 0_1ins258 |
| 7 | human | 112609 | MRAP2 | melanocortin 2 receptor acc... | XM_024446318.1 | 51.1% | 34.1% | (many diffs) |
| 8 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | NM_001101482.2 | 85.5% | 86.9% | (many diffs) |
| 9 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | NM_001177731.1 | 85.5% | 86.9% | (many diffs) |
| 10 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511174.2 | 85.5% | 86.9% | (many diffs) |
| 11 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511175.2 | 85.5% | 86.9% | (many diffs) |
| 12 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511176.2 | 85.5% | 86.9% | (many diffs) |
| 13 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511177.3 | 85.5% | 86.9% | (many diffs) |
| 14 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511178.3 | 85.5% | 86.9% | (many diffs) |
| 15 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511179.2 | 85.5% | 86.9% | (many diffs) |
| 16 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511180.3 | 85.5% | 86.9% | (many diffs) |
| 17 | mouse | 244958 | Mrap2 | melanocortin 2 receptor acc... | XM_006511173.3 | 83.1% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgcccagagg ttaatttcta acagaacctc ccagcaatcg gcatctaatt 121 ctgattacac ctgggaatat gaatattatg agattggacc agtttccttt gaaggactga 181 aggctcataa atattccatt gtgattggat tttgggttgg tcttgcagtc ttcgtgattt 241 ttatgttttt tgtgctgacc ttgctgacca agacaggagc cccacaccaa gacaatgcag 301 agtccTCAGA GAAGAGATTC AGAATGAACA GCTTTGTGTC AGACTTTGGA AGACCTCTGG 361 AGCCAGATAA AGTATTTTCT CGCCAAGGCA ACGAGGAGTC CAGGTCTCTC TTTCACTGCT 421 ACATCAATGA GGTGGAACGC TTGGACAGAG CCAAAGCTTG TCACCAGACC ACAGCCCTTG 481 ACAGTGACGT CCAACTCCAG GAAGCCATCA GAAGCAGTGG GCAGCCAGAG GAGGAGCTGA 541 ACAGGCTCAT GAAGTTTGAC ATCCCCAACT TTGTGAACAC AGACCAGAAC TACTTTGGGG 601 AGGATGATCT TCTGATTTCT GAACCACCTA TTGTTCTGGA AACTAAGCCA CTTTCCCAGA 661 CCTCACACAA AGACCTGGAT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCC TGAACCAGGA 841 TCTACTAACG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt