Construct: ORF TRCN0000468660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012228.1_s317c1
- Derived from:
- ccsbBroadEn_01787
- DNA Barcode:
- CCTCACAGCATATCTTCAATGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YES1 (7525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468660
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | NM_005433.4 | 99.9% | 100% | 1444T>C |
| 2 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_017025960.2 | 99.9% | 100% | 1444T>C |
| 3 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451243.1 | 99.9% | 100% | 1444T>C |
| 4 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451244.1 | 99.9% | 100% | 1444T>C |
| 5 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451245.1 | 99.9% | 100% | 1444T>C |
| 6 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451246.1 | 99.9% | 100% | 1444T>C |
| 7 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_005258139.4 | 91.8% | 91.8% | 1289_1290ins132;1312T>C |
| 8 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451247.1 | 91.8% | 91.8% | 1289_1290ins132;1312T>C |
| 9 | mouse | 22612 | Yes1 | YES proto-oncogene 1, Src f... | NM_001205132.1 | 91.1% | 95.9% | (many diffs) |
| 10 | mouse | 22612 | Yes1 | YES proto-oncogene 1, Src f... | NM_001205133.1 | 91.1% | 95.9% | (many diffs) |
| 11 | mouse | 22612 | Yes1 | YES proto-oncogene 1, Src f... | NM_009535.3 | 91.1% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1695
- ORF length:
- 1629
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcattaaa agtaaagaaa acaaaagtcc agccattaaa tacagacctg 121 aaaatactcc agagcctgtc agtacaagtg tgagccatta tggagcagaa cccactacag 181 tgtcaccatg tccgtcatct tcagcaaagg gaacagcagt taatttcagc agtctttcca 241 tgacaccatt tggaggatcc tcaggggtaa cgccttttgg aggtgcatct tcctcatttt 301 cagtggtgcc aagttcatat cctgctggtt taacaggtgg tgttactata tttgtggcct 361 tatatgatta tgaagctaga actacagaag acctttcatt taagaagggt gaaagatttc 421 aaataattaa caatacggaa ggagattggt gggaagcaag atcaatcgct acaggaaaga 481 atggttatat cccgagcaat tatgtagcgc ctgcagattc cattcaggca gaagaatggt 541 attttggcaa aatggggaga aaagatgctg aaagattact tttgaatcct ggaaatcaac 601 gaggtatttt cttagtaaga gagagtgaaa caactaaagg tgcttattcc ctttctattc 661 gtgattggga tgagataagg ggtgacaatg tgaaacacta caaaattagg aaacttgaca 721 atggtggata ctatatcaca accagagcac aatttgatac tctgcagaaa ttggtgaaac 781 actacacaga acatgctgat ggtttatgcc acaagttgac aactgtgtgt ccaactgtga 841 aacctcagac tcaaggtcta gcaaaagatg cttgggaaat ccctcgagaa tctttgcgac 901 tagaggttaa actaggacaa ggatgtttcg gcgaagtgtg gatgggaaca tggaatggaa 961 ccacgaaagt agcaatcaaa acactaaaac caggtacaat gatgccagaa gctttccttc 1021 aagaagctca gataatgaaa aaattaagac atgataaact tgttccacta tatgctgttg 1081 tttctgaaga accaatttac attgtcactg aatttatgtc aaaaggaagc ttattagatt 1141 tccttaagga aggagatgga aagtatttga agcttccaca gctggttgat atggctgctc 1201 agattgctga tggtatggca tatattgaaa gaatgaacta tattcaccga gatcttcggg 1261 ctgctaatat tcttGTAGGA GAAAATCTTG TGTGCAAAAT AGCAGACTTT GGTTTAGCAA 1321 GGTTAATTGA AGACAATGAA TACACAGCAA GACAAGGTGC AAAATTTCCA ATCAAATGGA 1381 CAGCTCCTGA AGCTGCACTG TATGGTCGGT TTACAATAAA GTCTGATGTC TGGTCATTTG 1441 GAATTCTGCA AACAGAACTA GTAACAAAGG GCCGAGTGCC ATATCCAGGT ATGGTGAACC 1501 GTGAAGTACT AGAACAAGTG GAGCGAGGAT ACAGGATGCC GTGCCCTCAG GGCTGTCCAG 1561 AATCCCTCCA TGAATTGATG AATCTGTGTT GGAAGAAGGA CCCTGATGAA AGACCAACAT 1621 TTGAATATAT TCAGTCCTTC TTGGAAGACT ACTTCACTGC TACAGAGCCA CAGTACCAGC 1681 CAGGAGAAAA TTTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1741 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1801 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTCACAGCA TATCTTCAAT 1861 GCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt