Construct: ORF TRCN0000468664

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003525.1_s317c1
Derived from:
ccsbBroadEn_01138
DNA Barcode:
CCATCTATCAACAGGTAATGGCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
P4HB (5034)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468664
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5034 P4HB prolyl 4-hydroxylase subuni... NM_000918.4 100% 100%
2 human 5034 P4HB prolyl 4-hydroxylase subuni... XM_024450777.1 91.4% 69.6% 1178_1305del;1638_1639insTGAAAGATGAACTG
3 human 5034 P4HB prolyl 4-hydroxylase subuni... XR_002958014.1 54.9% 1_64del;1423_1752del;1919_2774del
4 mouse 18453 P4hb prolyl 4-hydroxylase, beta ... NM_011032.2 86.4% 93.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1590
ORF length:
1524
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gcgccgcgct ctgctgtgcc tggccgtggc cgccctggtg cgcgccgacg
121cccccgagga ggaggaccac gtcctggtgc tgcggaaaag caacttcgcg gaggcgctgg
181cggcccacaa gtacctgctg gtggagttct atgccccttg gtgtggccac tgcaaggctc
241tggcccctga gtatgccaaa gccgctggga agctgaaggc agaaggttcc gagatcaggt
301tggccaaggt ggacgccacg gaggagtctg acctggccca gcagtacggc gtgcgcggct
361atcccaccat caagttcttc aggaatggag acacggcttc ccccaaggaa tatacagctg
421gcagagaggc tgatgacatc gtgaactggc tgaagaagcg cacgggcccg gctgccacca
481ccctgcctga cggcgcagct gcagagtcct tggtggagtc cagcgaggtg gctgtcatcg
541gcttcttcaa ggacgtggag tcggactctg ccaagcagtt tttgcaggca gcagaggcca
601tcgatgacat accatttggg atcacttcca acagtgacgt gttctccaaa taccagctcg
661acaaagatgg ggttgtcctc tttaagaagt ttgatgaagg ccggaacaac tttgaagggg
721aggtcaccaa ggagaacctg ctggacttta tcaaacacaa ccagctgccc cttgtcatcg
781agttcaccga gcagacagcc ccgaagattt ttggaggtga aatcaagact cacatcctgc
841tgttcttgcc caagagtgtg tctgactatg acggcaaact gagcaacttc aaaacagcag
901ccgagagctt caagggcaag atcctgttca tcttcatcga cagcgaccac accgacaacc
961agcgcatcct cgagttcttt ggcctgaaga aggaagagtg cccggccgtg cgcctcatca
1021ccctggagga ggagatgacc aagtacaagc ccgaatcgga ggagctgacg gcagagagga
1081tcacagagtt ctgccaccgc ttcctggagg gcaaaatcaa gccccaccTG ATGAGCCAGG
1141AGCTGCCGGA GGACTGGGAC AAGCAGCCTG TCAAGGTGCT TGTTGGGAAG AACTTTGAAG
1201ACGTGGCTTT TGATGAGAAA AAAAACGTCT TTGTGGAGTT CTATGCCCCA TGGTGTGGTC
1261ACTGCAAACA GTTGGCTCCC ATTTGGGATA AACTGGGAGA GACGTACAAG GACCATGAGA
1321ACATCGTCAT CGCCAAGATG GACTCGACTG CCAACGAGGT GGAGGCCGTC AAAGTGCACA
1381GCTTCCCCAC ACTCAAGTTC TTTCCTGCCA GTGCCGACAG GACGGTCATT GATTACAACG
1441GGGAACGCAC GCTGGATGGT TTTAAGAAAT TCCTGGAGAG CGGTGGCCAG GATGGGGCAG
1501GGGATGATGA CGATCTCGAG GACCTGGAAG AAGCAGAGGA GCCAGACATG GAGGAAGACG
1561ATGATCAGAA AGCTGTGAAA GATGAACTGT ACCCAACTTC CTTGTACAAA GTGGTTGATA
1621TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1681CGTAACTTAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCATCT
1741ATCAACAGGT AATGGCCAAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
1801gaaagatt

Download FASTA (ORF) (Full)