Construct: ORF TRCN0000468666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012862.1_s317c1
- Derived from:
- ccsbBroadEn_05803
- DNA Barcode:
- TACTAGCAATCATCCCTGTGACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH3B2 (222)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222 | ALDH3B2 | aldehyde dehydrogenase 3 fa... | NM_001031615.2 | 99.7% | 99.4% | 608A>G;826C>T;1146C>T |
| 2 | human | 222 | ALDH3B2 | aldehyde dehydrogenase 3 fa... | NM_001354345.1 | 99.7% | 99.4% | 608A>G;826C>T;1146C>T |
| 3 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001030010.3 | 77.5% | 72.8% | (many diffs) |
| 4 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_000694.4 | 71.3% | 68.5% | (many diffs) |
| 5 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001161473.3 | 71.3% | 68.5% | (many diffs) |
| 6 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001290059.2 | 61% | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggatgaacca cggtccacga acctgttcat gaagctggac tcggtcttca 121 tctggaagga accctttggc ctggtcctca tcatcgcacc ctggaactac ccactgaacc 181 tgaccctggt gctcctggtg ggcgccctcg ccgcagggag ttgcgtggtg ctgaagccgt 241 cagaaatcag ccagggcaca gagaaggtcc tggctgaggt gctgccccag tacctggacc 301 agagctgctt tgccgtggtg ctgggcggac cccaggagac agggcagctg ctagagcaca 361 agttggacta catcttcttc acagggagcc ctcgtgtggg caagattgtc atgactgctg 421 ccaccaagca cctgacgcct gtcaccctgg agctgggggg caagaacccc tgctacgtgg 481 acgacaactg cgacccccag accgtggcca accgcgtggc ctggttctgc tacttcaatg 541 ccggccagac ctgcgtggcc cctgactacg tcctgtgcag ccccgagatg caggagaggc 601 tgctgcccgc cctgcagagc accatcaccc gtttctatgg cgacgacccc cagagctccc 661 caaacctggg ccgcatcatc aaccagaaac agttccagcg gctgcgggca ttgctgggct 721 gcggccgcgt ggccattggg ggccagagca acgagagcga tcgctacatc gcccccacgg 781 tgctggtgga cgtgcaggag acggagcctg tgatgcagga ggagatcttc gggcccatcc 841 tgcccatcgt gaacgtgcag agcgtggacg aggccatcaa gttcatcaac tggcaggaga 901 agcccctggc cctgtacgcc ttctccaaca gcagccaggt tgtgaaccag atgctggagc 961 ggaccagcag cggcagcttt ggaggcaatg agggcttcac ctacatatct ctgctgtccg 1021 tgccattcgg gggagtcggc cacagtggga tgggccggta ccacggcaag ttcaccttcg 1081 acaccttctc ccaccaccgc acctgcctgc tcgccccctc cggcctggag aaattaaagg 1141 agatccacta cccaccctat accgactgga accagcagct gttacgctgg ggcatgggct 1201 cccagagctg tacccTCCTG TGCCCAACTA TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACT AGCAATCATC 1381 CCTGTGACTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt