Construct: ORF TRCN0000468669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002705.1_s317c1
- Derived from:
- ccsbBroadEn_06951
- DNA Barcode:
- TGGCTCCAGTACTTTTATCGAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIX1 (6495)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468669
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6495 | SIX1 | SIX homeobox 1 | NM_005982.4 | 99.8% | 99.6% | 295C>T |
| 2 | human | 6495 | SIX1 | SIX homeobox 1 | XM_017021602.2 | 60.7% | 59.1% | 295C>T;499_500ins59;519_520ins274 |
| 3 | mouse | 20471 | Six1 | sine oculis-related homeobox 1 | NM_009189.3 | 93.1% | 98.9% | (many diffs) |
| 4 | mouse | 20472 | Six2 | sine oculis-related homeobox 2 | XM_011246335.2 | 61.4% | 65.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gatgctgccg tcgtttggct ttacgcagga gcaagtggcg tgcgtgtgcg 121 aggttctgca gcaaggcgga aacctggagc gcctgggcag gttcctgtgg tcactgcccg 181 cctgcgacca cctgcacaag aacgagagcg tactcaaggc caaggcggtg gtcgccttcc 241 accgcggcaa cttccgtgag ctctacaaga tcctggagag ccaccagttc tcgcctcaca 301 accaccccaa actgcagcaa ctgtggctga aggcgcatta cgtggaggcc gagaagctgt 361 gcggccgacc cctgggcgcc gtgggcaaat atcgggtgcg ccgaaaattt ccactgccgc 421 gcaccatctg ggacggcgag gagaccagct actgcttcaa ggagaagtcg aggggtgtcc 481 tgcgggagtg gtacgcgcac aatccctacc catcgccgcg tgagaagcgg gagctggccg 541 aggccaccgg cctcaccacc acccaggtca gcaactggtt taagaaccgg aggcaaagag 601 accgggccgc ggaggccaag gaaagggaga acaccgaaaa caataactcc tcctccaaca 661 agcagaacca actctctcct ctggaagggg gcaagccgct catgtccagc tcagaagagg 721 aattctcacc tccccaaagt ccagaccaga actcggtcct tctgctgcag ggcaatatgg 781 gccacgccag gagctcaaac tattctctcc cgggcttaac agcctcgcag cccagtcacg 841 gcctgcagac ccaccagcat cagctccaag actcTCTGCT CGGCCCCCTC ACCTCCAGTC 901 TGGTGGACTT GGGGTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGCCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGCTCC AGTACTTTTA 1081 TCGAAGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt