Construct: ORF TRCN0000468672
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016684.1_s317c1
- Derived from:
- ccsbBroadEn_15599
- DNA Barcode:
- ACCATTACCCTAGGTCTATTGGAA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRP54 (6729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468672
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6729 | SRP54 | signal recognition particle 54 | NM_003136.4 | 99.9% | 98.6% | 1497delG |
| 2 | human | 6729 | SRP54 | signal recognition particle 54 | XM_011537106.1 | 99.9% | 98.6% | 1497delG |
| 3 | human | 6729 | SRP54 | signal recognition particle 54 | NM_001146282.1 | 90.2% | 87.5% | 0_1ins55;23_24ins92;1350delG |
| 4 | human | 6729 | SRP54 | signal recognition particle 54 | XM_005268024.3 | 88.6% | 87.3% | 1153_1154ins171;1326delG |
| 5 | human | 6729 | SRP54 | signal recognition particle 54 | XM_017021615.2 | 79.2% | 78% | 0_1ins312;1185delG |
| 6 | mouse | 665155 | Srp54b | signal recognition particle... | NM_001100109.1 | 92.1% | 98.4% | (many diffs) |
| 7 | mouse | 24067 | Srp54a | signal recognition particle... | NM_011899.4 | 92.1% | 98.4% | (many diffs) |
| 8 | mouse | 100101806 | Srp54c | signal recognition particle... | NM_001100110.1 | 91.7% | 97.6% | (many diffs) |
| 9 | mouse | 100101806 | Srp54c | signal recognition particle... | XM_006515318.3 | 87.4% | 93.6% | (many diffs) |
| 10 | mouse | 24067 | Srp54a | signal recognition particle... | XM_006515868.3 | 65.9% | 70.5% | (many diffs) |
| 11 | mouse | 665155 | Srp54b | signal recognition particle... | XM_006516155.3 | 65.9% | 70.5% | (many diffs) |
| 12 | mouse | 100101806 | Srp54c | signal recognition particle... | XM_011243971.2 | 65.5% | 69.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tctagcagac cttggaagaa aaataacatc agcattacgc tcgttgagca 121 atgccaccat tatcaatgaa gaggtattga atgctatgct aaaagaagtc tgtaccgctt 181 tgttggaagc agatgttaat attaaactag tgaagcaact aagagaaaat gttaagtctg 241 ctattgatct tgaagagatg gcatctggtc ttaacaaaag aaaaatgatt cagcatgctg 301 tatttaaaga acttgtgaag cttgtagacc ctggagttaa ggcatggaca cccactaaag 361 gaaaacaaaa tgtgattatg tttgttggat tgcaagggag tggtaaaaca acaacatgtt 421 caaagctagc atattattac cagaggaaag gttggaagac ctgtttaata tgtgcagaca 481 cattcagagc aggggctttt gaccaactaa aacagaatgc taccaaagca agaattccat 541 tttatggaag ctatacagaa atggatcctg tcatcattgc ttctgaagga gtagagaaat 601 ttaaaaatga aaattttgaa attattattg ttgatacaag tggccgccac aaacaagaag 661 actctttgtt tgaagaaatg cttcaagttg ctaatgctat acaacctgat aacattgttt 721 atgtgatgga tgcctccatt gggcaggctt gtgaagccca ggctaaggct tttaaagata 781 aagtagatgt agcctcagta atagtgacaa aacttgatgg ccatgcaaaa ggaggtggtg 841 cactcagtgc agtcgctgcc acaaaaagtc cgattatttt cattggtaca ggggaacata 901 tagatgactt tgaacctttc aaaacacagc cttttattag caaacttctt ggtatgggcg 961 acattgaagg actgatagat aaagtcaacg agttgaagtt ggatgacaat gaagcactta 1021 tagagaagtt gaaacatggt cagtttacgt tgcgagacat gtatgagcaa tttcaaaata 1081 tcatgaaaat gggccccttc agtcagatct tggggatgat CCCTGGTTTT GGGACAGATT 1141 TTATGAGCAA AGGAAATGAA CAGGAGTCAA TGGCAAGGCT AAAGAAATTA ATGACAATAA 1201 TGGATAGTAT GAATGATCAA GAACTAGACA GTACGGATGG TGCCAAAGTT TTTAGTAAAC 1261 AACCAGGAAG AATCCAAAGA GTAGCAAGAG GATCGGGTGT ATCAACAAGA GATGTTCAAG 1321 AACTTTTGAC ACAATATACC AAGTTTGCAC AGATGGTAAA AAAGATGGGA GGTATCAAAG 1381 GACTTTTCAA AGGTGGCGAC ATGTCTAAGA ATGTGAGCCA GTCACAGATG GCAAAATTGA 1441 ACCAACAAAT GGCCAAAATG ATGGATCCTA GGGTTCTTCA TCACATGGGT GGTATGGCAG 1501 GACTTCAGTC AATGATGAGG CAGTTTCAAC AGGGTGCTGC TGGCAACATG AAAGGCATGA 1561 TGGATTCAAT AATATGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 1621 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 1681 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCATTAC CCTAGGTCTA 1741 TTGGAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt