Construct: ORF TRCN0000468685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017538.1_s317c1
- Derived from:
- ccsbBroadEn_02831
- DNA Barcode:
- TTGAAGTCAGGACCAGACGTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGK3 (23678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468685
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23678 | SGK3 | serum/glucocorticoid regula... | NM_001033578.3 | 100% | 100% | |
| 2 | human | 100533105 | C8orf44-SGK3 | C8orf44-SGK3 readthrough | NM_001204173.2 | 100% | 100% | |
| 3 | human | 23678 | SGK3 | serum/glucocorticoid regula... | NM_013257.4 | 100% | 100% | |
| 4 | human | 23678 | SGK3 | serum/glucocorticoid regula... | NM_170709.3 | 93.5% | 93.5% | 977_978ins96 |
| 5 | mouse | 170755 | Sgk3 | serum/glucocorticoid regula... | NM_001037759.1 | 90.3% | 96.7% | (many diffs) |
| 6 | mouse | 170755 | Sgk3 | serum/glucocorticoid regula... | NM_133220.2 | 90.3% | 96.7% | (many diffs) |
| 7 | mouse | 170755 | Sgk3 | serum/glucocorticoid regula... | NM_177547.3 | 90.3% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1554
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca aagagatcac accatggact acaaggaaag ctgcccaagt gtaagcattc 121 ccagctccga tgaacacaga gagaaaaaga agaggtttac tgtttataaa gttctggttt 181 cagtgggaag aagtgaatgg tttgtcttca ggagatatgc agagtttgat aaactttata 241 acactttaaa aaaacagttt cctgctatgg ccctgaagat tcctgccaag agaatatttg 301 gtgataattt tgatccagat tttattaaac aaagacgagc aggactaaac gaattcattc 361 agaacctagt taggtatcca gaactttata accatccaga tgtcagagca ttccttcaaa 421 tggacagtcc aaaacaccag tcagatccat ctgaagatga ggatgaaaga agttctcaga 481 agctacactc tacctcacag aacatcaacc tgggaccgtc tggaaatcct catgccaaac 541 caactgactt tgatttctta aaagttattg gaaaaggcag ctttggcaag gttcttcttg 601 caaaacggaa actggatgga aaattttatg ctgtcaaagt gttacagaaa aaaatagttc 661 tcaacagaaa agagcaaaaa catattatgg ctgaacgtaa tgtgctcttg aaaaatgtga 721 aacatccgtt tttggttgga ttgcattatt ccttccaaac aactgaaaag ctttattttg 781 ttctggattt tgttaatgga ggggagcttt ttttccactt acaaagagaa cggtcctttc 841 ctgagcacag agctaggttt tacgctgctg aaattgctag tgcattgggt tacttacatt 901 ccatcaaaat agtatacaga gacttgaaac cagaaaatat tcttttggat tcagtaggac 961 atgttgtctt aacagatttt gggctttgta aagaaggaat tgctatttct gacaccacta 1021 ccacattttg tgggacacca gagtatcttg cacctgaagt aattagaaaa cagccctatg 1081 acaatactgt agattggtgg tgccttgggg ctgttctgta tgaaatgctg tatggattgc 1141 ctccttttta ttgccgagat gttgctgaaa tgtatgacaa tatccttcac aaacccctaa 1201 gtttGAGGCC AGGAGTGAGT CTTACAGCCT GGTCCATTCT GGAAGAACTC CTAGAAAAAG 1261 ACAGGCAAAA TCGACTTGGT GCCAAGGAAG ACTTTCTTGA AATTCAGAAT CATCCTTTTT 1321 TTGAATCACT CAGCTGGGCT GACCTTGTAC AAAAGAAGAT TCCACCACCA TTTAATCCTA 1381 ATGTGGCTGG ACCAGATGAT ATCAGAAACT TTGACACAGC ATTTACAGAA GAAACAGTTC 1441 CATATTCTGT GTGTGTATCT TCTGACTATT CTATAGTGAA TGCCAGTGTA TTGGAGGCAG 1501 ATGATGCATT CGTTGGTTTC TCTTATGCAC CTCCTTCAGA AGACTTATTT TTGTGCCCAA 1561 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1621 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1681 TATCTTGTGG AAAGGACGAT TGAAGTCAGG ACCAGACGTT ATTACGCGTT AAGTCgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag att