Construct: ORF TRCN0000468685

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017538.1_s317c1
Derived from:
ccsbBroadEn_02831
DNA Barcode:
TTGAAGTCAGGACCAGACGTTATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SGK3 (23678)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468685
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23678 SGK3 serum/glucocorticoid regula... NM_001033578.3 100% 100%
2 human 100533105 C8orf44-SGK3 C8orf44-SGK3 readthrough NM_001204173.2 100% 100%
3 human 23678 SGK3 serum/glucocorticoid regula... NM_013257.4 100% 100%
4 human 23678 SGK3 serum/glucocorticoid regula... NM_170709.3 93.5% 93.5% 977_978ins96
5 mouse 170755 Sgk3 serum/glucocorticoid regula... NM_001037759.1 90.3% 96.7% (many diffs)
6 mouse 170755 Sgk3 serum/glucocorticoid regula... NM_133220.2 90.3% 96.7% (many diffs)
7 mouse 170755 Sgk3 serum/glucocorticoid regula... NM_177547.3 90.3% 96.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1554
ORF length:
1488
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca aagagatcac accatggact acaaggaaag ctgcccaagt gtaagcattc
121ccagctccga tgaacacaga gagaaaaaga agaggtttac tgtttataaa gttctggttt
181cagtgggaag aagtgaatgg tttgtcttca ggagatatgc agagtttgat aaactttata
241acactttaaa aaaacagttt cctgctatgg ccctgaagat tcctgccaag agaatatttg
301gtgataattt tgatccagat tttattaaac aaagacgagc aggactaaac gaattcattc
361agaacctagt taggtatcca gaactttata accatccaga tgtcagagca ttccttcaaa
421tggacagtcc aaaacaccag tcagatccat ctgaagatga ggatgaaaga agttctcaga
481agctacactc tacctcacag aacatcaacc tgggaccgtc tggaaatcct catgccaaac
541caactgactt tgatttctta aaagttattg gaaaaggcag ctttggcaag gttcttcttg
601caaaacggaa actggatgga aaattttatg ctgtcaaagt gttacagaaa aaaatagttc
661tcaacagaaa agagcaaaaa catattatgg ctgaacgtaa tgtgctcttg aaaaatgtga
721aacatccgtt tttggttgga ttgcattatt ccttccaaac aactgaaaag ctttattttg
781ttctggattt tgttaatgga ggggagcttt ttttccactt acaaagagaa cggtcctttc
841ctgagcacag agctaggttt tacgctgctg aaattgctag tgcattgggt tacttacatt
901ccatcaaaat agtatacaga gacttgaaac cagaaaatat tcttttggat tcagtaggac
961atgttgtctt aacagatttt gggctttgta aagaaggaat tgctatttct gacaccacta
1021ccacattttg tgggacacca gagtatcttg cacctgaagt aattagaaaa cagccctatg
1081acaatactgt agattggtgg tgccttgggg ctgttctgta tgaaatgctg tatggattgc
1141ctccttttta ttgccgagat gttgctgaaa tgtatgacaa tatccttcac aaacccctaa
1201gtttGAGGCC AGGAGTGAGT CTTACAGCCT GGTCCATTCT GGAAGAACTC CTAGAAAAAG
1261ACAGGCAAAA TCGACTTGGT GCCAAGGAAG ACTTTCTTGA AATTCAGAAT CATCCTTTTT
1321TTGAATCACT CAGCTGGGCT GACCTTGTAC AAAAGAAGAT TCCACCACCA TTTAATCCTA
1381ATGTGGCTGG ACCAGATGAT ATCAGAAACT TTGACACAGC ATTTACAGAA GAAACAGTTC
1441CATATTCTGT GTGTGTATCT TCTGACTATT CTATAGTGAA TGCCAGTGTA TTGGAGGCAG
1501ATGATGCATT CGTTGGTTTC TCTTATGCAC CTCCTTCAGA AGACTTATTT TTGTGCCCAA
1561CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1621ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1681TATCTTGTGG AAAGGACGAT TGAAGTCAGG ACCAGACGTT ATTACGCGTT AAGTCgacaa
1741tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)