Transcript: Human NM_001033578.3

Homo sapiens serum/glucocorticoid regulated kinase family member 3 (SGK3), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
SGK3 (23678)
Length:
4106
CDS:
175..1665

Additional Resources:

NCBI RefSeq record:
NM_001033578.3
NBCI Gene record:
SGK3 (23678)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147184 ACCTCACAGAACATCAACCT pXPR_003 GGG 443 30% 7 0.3288 C8orf44-SGK3, SGK3 SGK3 75542
2 BRDN0001147093 CTCTGGTGTCCCACAAAATG pXPR_003 TGG 961 64% 13 0.1431 C8orf44-SGK3, SGK3 C8orf44-SGK3 75907
3 BRDN0001146019 TGTCCCACAAAATGTGGTAG pXPR_003 TGG 955 64% 13 -0.4714 C8orf44-SGK3, SGK3 SGK3 75540
4 BRDN0001146281 TTATAAAGTTCTGGTTTCAG pXPR_003 TGG 115 8% 3 -0.8127 C8orf44-SGK3, SGK3 SGK3 75541
5 BRDN0001146567 GCTGAAATTGCTAGTGCATT pXPR_003 GGG 818 55% 11 -0.8309 C8orf44-SGK3, SGK3 C8orf44-SGK3 75906
6 BRDN0001148559 TTTATTAAACAAAGACGAGC pXPR_003 AGG 272 18% 5 -0.8922 C8orf44-SGK3, SGK3 C8orf44-SGK3 75908
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001033578.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195193 CAGTAGGACATGTTGTCTTAA pLKO.1 1061 CDS 100% 13.200 6.600 Y SGK3 n/a
2 TRCN0000195153 CCTTTAGAGGTAAGACTTTAA pLKO.1 3831 3UTR 100% 13.200 6.600 Y SGK3 n/a
3 TRCN0000001520 GCATTCGTTGGTTTCTCTTAT pLKO.1 1615 CDS 100% 13.200 6.600 Y SGK3 n/a
4 TRCN0000280065 GCATTCGTTGGTTTCTCTTAT pLKO_005 1615 CDS 100% 13.200 6.600 Y SGK3 n/a
5 TRCN0000194666 CCAACTAACTTACATGCTTAT pLKO.1 3367 3UTR 100% 10.800 5.400 Y SGK3 n/a
6 TRCN0000194966 CCACCACCATTTAATCCTAAT pLKO.1 1471 CDS 100% 10.800 5.400 Y SGK3 n/a
7 TRCN0000195023 CTGACTATTCTATAGTGAATG pLKO.1 1571 CDS 100% 10.800 5.400 Y SGK3 n/a
8 TRCN0000196412 GCAGATCATTTAAGAAGTAAC pLKO.1 2235 3UTR 100% 10.800 5.400 Y SGK3 n/a
9 TRCN0000196501 GTTCATGGTATGATCGAAATG pLKO.1 2159 3UTR 100% 10.800 5.400 Y SGK3 n/a
10 TRCN0000280063 GTTCATGGTATGATCGAAATG pLKO_005 2159 3UTR 100% 10.800 5.400 Y SGK3 n/a
11 TRCN0000199867 GCTGCCCAAGTGTAAGCATTC pLKO.1 209 CDS 100% 6.000 3.000 Y SGK3 n/a
12 TRCN0000001519 GAACGTAATGTGCTCTTGAAA pLKO.1 802 CDS 100% 5.625 2.813 Y SGK3 n/a
13 TRCN0000001521 GCCGAGATGTTGCTGAAATGT pLKO.1 1262 CDS 100% 5.625 2.813 Y SGK3 n/a
14 TRCN0000010272 GCTGGGCTGACCTTGTACAAA pLKO.1 1442 CDS 100% 5.625 2.813 Y SGK3 n/a
15 TRCN0000280066 GCTGGGCTGACCTTGTACAAA pLKO_005 1442 CDS 100% 5.625 2.813 Y SGK3 n/a
16 TRCN0000010281 GGTTACTTACATTCCATCAAA pLKO.1 997 CDS 100% 5.625 2.813 Y SGK3 n/a
17 TRCN0000010273 ATTCGTTGGTTTCTCTTATGC pLKO.1 1617 CDS 100% 4.950 2.475 Y SGK3 n/a
18 TRCN0000194738 CCAAGAGAATATTTGGTGATA pLKO.1 395 CDS 100% 4.950 2.475 Y SGK3 n/a
19 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2276 3UTR 100% 4.950 2.475 Y ERAP2 n/a
20 TRCN0000001517 GCGAGACCCTAGTTAAGAGAA pLKO.1 3194 3UTR 100% 4.950 2.475 Y SGK3 n/a
21 TRCN0000022746 GCTGTCAAAGTGTTACAGAAA pLKO.1 739 CDS 100% 4.950 2.475 Y Sgk3 n/a
22 TRCN0000297999 GCTGTCAAAGTGTTACAGAAA pLKO_005 739 CDS 100% 4.950 2.475 Y Sgk3 n/a
23 TRCN0000022748 GCCAAGAGAATATTTGGTGAT pLKO.1 394 CDS 100% 4.050 2.025 Y Sgk3 n/a
24 TRCN0000010282 GGCTGAACGTAATGTGCTCTT pLKO.1 798 CDS 100% 4.050 2.025 Y SGK3 n/a
25 TRCN0000297753 GGCTGAACGTAATGTGCTCTT pLKO_005 798 CDS 100% 4.050 2.025 Y SGK3 n/a
26 TRCN0000001518 GCAGGACTAAACGAATTCATT pLKO.1 448 CDS 100% 0.000 0.000 Y SGK3 n/a
27 TRCN0000010274 GCAGGACTAAACGAATTCATT pLKO.1 448 CDS 100% 0.000 0.000 Y SGK3 n/a
28 TRCN0000280064 GCAGGACTAAACGAATTCATT pLKO_005 448 CDS 100% 0.000 0.000 Y SGK3 n/a
29 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2277 3UTR 100% 13.200 6.600 Y LIAS n/a
30 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2442 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001033578.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02831 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02831 pLX_304 13.3% 100% 100% V5 n/a
3 TRCN0000468685 TTGAAGTCAGGACCAGACGTTATT pLX_317 23.8% 100% 100% V5 n/a
4 ccsbBroadEn_15022 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_15022 pLX_304 13.3% 100% 100% V5 n/a
6 TRCN0000469217 ACGGTTCGTGCAACGCCATGTATG pLX_317 22.2% 100% 100% V5 n/a
7 TRCN0000488506 CGATGACTGCGAGTCCATCGGAGT pLX_317 17% 100% 100% V5 (not translated due to prior stop codon) n/a
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