Construct: ORF TRCN0000468687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017705.1_s317c1
- Derived from:
- ccsbBroadEn_02611
- DNA Barcode:
- ATTCATAACATCGCCTGACCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM115 (11070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11070 | TMEM115 | transmembrane protein 115 | NM_007024.5 | 100% | 100% | |
| 2 | mouse | 56395 | Tmem115 | transmembrane protein 115 | NM_019704.2 | 87.9% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca acgtgccctg ccaggcgccc gccagcactt gggggccatt ctggccagcg 121 ccagcgtggt ggtgaaggct ctgtgtgcgg cggtactatt cctctacctg ctctccttcg 181 ccgtggacac aggctgcctg gcggtcaccc cgggctacct ctttcctccc aacttctgga 241 tctggaccct ggccacccat gggctgatgg agcagcatgt gtgggacgtg gccatcagcc 301 tgacaacggt ggtggtggcc gggcgtttgc tggagcccct ctggggggcc ttggagctgc 361 tcatcttctt ctcagtggtg aatgtgtctg tagggctgct gggggccttc gcctacctcc 421 tcacctacat ggcttccttc aacctggtct acctgttcac tgtccgtatc cacggcgcct 481 tgggcttcct aggtggcgtc ctggtggcac tcaagcaaac catgggggac tgtgtggtcc 541 tgcgagtgcc ccaggtgcgc gtcagtgtga tgcccatgct gctgctggcg ctgctgctcc 601 tgctgcggct cgccacgctg ctccagagcc cggcgctggc ttcctatggc ttcgggctgc 661 tctccagttg ggtatatctt cgcttctacc agcgccatag ccggggccga ggggacatgg 721 ctgaccactt tgctttcgcc actttcttcc ctgagatcct gcagcctgtg gtgggtttgc 781 tggcgaactt ggtgcacagc ctcctggtga aggtaaagat atgccagaag acggtgaagc 841 gcTACGATGT GGGTGCCCCA TCCTCCATCA CCATCAGCCT GCCAGGCACA GACCCTCAAG 901 ACGCCGAGCG GAGAAGGCAA CTGGCCCTGA AGGCACTCAA TGAGCGGCTG AAGAGAGTGG 961 AAGACCAGTC CATCTGGCCC AGCATGGATG ATGATGAAGA GGAGTCTGGG GCCAAGGTGG 1021 ACAGCCCCCT GCCCTCAGAC AAAGCTCCCA CACCCCCAGG GAAGGGGGCT GCCCCAGAAT 1081 CCAGTCTAAT CACCTTCGAG GCAGCTCCCC CGACGCTGTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAATTCAT AACATCGCCT GACCAGTCAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt