Construct: ORF TRCN0000468691
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005659.1_s317c1
- Derived from:
- ccsbBroadEn_09223
- DNA Barcode:
- AGTACTCCTAGATACCCCTTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYROXD2 (84795)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468691
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | NM_032709.3 | 99.8% | 99.6% | 258C>T;1282C>T;1382T>C |
| 2 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016835.1 | 96% | 95.8% | (many diffs) |
| 3 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_011540293.2 | 91.8% | 88.9% | (many diffs) |
| 4 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747233.1 | 81.1% | (many diffs) | |
| 5 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747235.1 | 79.5% | (many diffs) | |
| 6 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016838.1 | 79% | 78.8% | 0_1ins363;919C>T;1019T>C |
| 7 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016839.2 | 79% | 78.8% | 0_1ins363;919C>T;1019T>C |
| 8 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016840.1 | 79% | 78.8% | 0_1ins363;919C>T;1019T>C |
| 9 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016841.1 | 79% | 78.8% | 0_1ins363;919C>T;1019T>C |
| 10 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747236.1 | 78.2% | (many diffs) | |
| 11 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747234.1 | 76.9% | (many diffs) | |
| 12 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747237.1 | 76.6% | (many diffs) | |
| 13 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016836.1 | 75.1% | 71.6% | (many diffs) |
| 14 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_017016837.1 | 75.1% | 71.6% | (many diffs) |
| 15 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747238.1 | 73.2% | (many diffs) | |
| 16 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747239.1 | 71.8% | (many diffs) | |
| 17 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XM_011540301.2 | 53.5% | 53.3% | 0_1ins807;475C>T;575T>C |
| 18 | human | 84795 | PYROXD2 | pyridine nucleotide-disulph... | XR_001747240.1 | 51.4% | (many diffs) | |
| 19 | human | 100302133 | MIR1287 | microRNA 1287 | NR_031619.1 | 4.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1809
- ORF length:
- 1743
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaagtggc cgaggtctct gcaaggctgt ggccgcctct cccttcccgg 121 cgtggagacg agataacacg gaagccaggg gaggtctgaa gcctgagtat gatgcggtgg 181 tgataggagc aggacacaac ggactggtgg ctgcagcgta cctgcagaga ctgggggtga 241 acaccgccgt cttcgagagg cgccatgtga tcgggggtgc agctgtcact gaggagatca 301 tcccagggtt taagttctcc cgtgcgtcct acctgctcag cctgctgagg ccgcagattt 361 acactgatct ggagctgaag aaacatgggc tgaggcttca tcttcgaaac ccctactcct 421 tcacccccat gctggaagag ggtgcaggca gcaaggtgcc caggtgcctt ctgctgggca 481 cagacatggc agaaaaccag aagcagatcg cccagttctc ccagaaggat gcccaggtct 541 ttcccaaata tgaggagttc atgcatcgct tggcattagc cattgaccct ctgctggatg 601 cggcccccgt ggacatggcg gccttccagc atggctcctt gctgcaaagg atgaggtcgc 661 tctccaccct caagcccctg ctgaaggcag gccgcatcct gggagcccag cttccccgat 721 attatgaggt cctcacagct cccattacca aggtgctgga tcagtggttc gagtctgagc 781 ctttaaaagc cactctagcc acagatgcag tgattggagc catgacaagt ccccacactc 841 cggggagtgg gtatgtgctg ctgcaccatg tgatgggggg cctggaggga atgcaggggg 901 cctggggcta cgtccagggg ggcatgggtg ccctctctga tgcgatcgca agctcagcca 961 ccacacatgg agcaagcatc ttcactgaaa agacagtggc gaaggtgcag gtgaacagtg 1021 aaggctgtgt tcaaggagtt gtgctggaag atggcacaga ggtgagaagc aaaatggtgc 1081 tgtccaacac atcaccgcag atcaccttcc tgaagctgac gccacaggag tggcttcctg 1141 aggagttcct ggagagaatc tctcagctgg acacccggtc gcctgtcacc aagatcaatg 1201 tggccgtaga caggctgccc agcttcctgg cggcccccaa tgctcccagg ggccagccgc 1261 tgccccatca ccaatgctcc atccacctga actgtgaaga caccctcctc cttcatcagg 1321 cctttgaaga tgccatggat ggcctgtctt cccacaggcc tgtgattgag ctctgcatcc 1381 cTTCCTCGCT GGACCCCACC CTGGCTCCCC CTGGCTGCCA TGTAGTCTCC CTCTTCACTC 1441 AGTACACGCC CTATACGCTG GCTGGAGGCA AGGCCTGGGA CGAGCAGGAG AGAGACGCTT 1501 ATGCAGACAG AGTGTTTGAT TGCATCGAGG TCTATGCCCC TGGCTTCAAG GACTCTGTGG 1561 TTGGCAGAGA CATCCTCACA CCACCAGATT TGGAGAGAAT CTTCGGGCTT CCTGGAGGGA 1621 ACATATTCCA CTGCGCCATG TCCCTGGACC AGCTCTACTT CGCCCGCCCC GTGCCCCTGC 1681 ATTCTGGCTA CCGCTGCCCT CTCCAGGGCC TGTATCTCTG TGGAAGTGGG GCTCATCCTG 1741 GAGGAGGTGT GATGGGAGCT GCTGGGCGAA ATGCAGCACA TGTGGCCTTT AGGGACCTCA 1801 AGAGCATGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1861 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1921 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTACT CCTAGATACC CCTTAACCAC 1981 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt