Construct: ORF TRCN0000468718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002594.1_s317c1
- Derived from:
- ccsbBroadEn_02314
- DNA Barcode:
- ACGTGCATACCCAGCACTGCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN5 (10098)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10098 | TSPAN5 | tetraspanin 5 | NM_005723.4 | 100% | 100% | |
| 2 | human | 10098 | TSPAN5 | tetraspanin 5 | XM_005262680.1 | 81.7% | 81.7% | 132_133ins147 |
| 3 | human | 10098 | TSPAN5 | tetraspanin 5 | XR_244617.1 | 21.7% | 1_435del;885_886ins64;1176_3333del | |
| 4 | human | 10098 | TSPAN5 | tetraspanin 5 | XR_001741092.1 | 17.4% | (many diffs) | |
| 5 | mouse | 56224 | Tspan5 | tetraspanin 5 | NM_019571.5 | 93.1% | 100% | (many diffs) |
| 6 | mouse | 56224 | Tspan5 | tetraspanin 5 | XM_006501736.3 | 74.8% | 78.5% | (many diffs) |
| 7 | mouse | 56224 | Tspan5 | tetraspanin 5 | XM_006501737.3 | 68.1% | 73.5% | (many diffs) |
| 8 | mouse | 56224 | Tspan5 | tetraspanin 5 | NR_121195.1 | 21.6% | (many diffs) | |
| 9 | mouse | 56224 | Tspan5 | tetraspanin 5 | XR_375551.3 | 11% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 870
- ORF length:
- 804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgggaagcac tacaagggtc ctgaagtcag ttgttgcatc aaatacttca 121 tatttggctt caatgtcata ttttggtttt tgggaataac atttcttgga attggactgt 181 gggcatggaa tgaaaaagga gttctgtcca acatctcttc catcaccgat ctcggcggct 241 ttgacccagt ttggctcttc cttgtggtgg gaggagtgat gttcattttg ggatttgcag 301 ggtgcattgg agcgctacgg gaaaacactt tccttctcaa gtttttttct gtgttcctgg 361 gaattatttt cttcctggag ctcactgccg gagttctagc atttgttttc aaagactgga 421 tcaaagacca gctgtatttc tttataaaca acaacatcag agcatatcgg gatgacattg 481 atttgcaaaa cctcatagac ttcacccagg aatattggca gtgctgtggg gcttttGGAG 541 CTGATGATTG GAACCTAAAT ATTTACTTCA ATTGCACAGA TTCCAATGCA AGTCGAGAGC 601 GATGTGGCGT TCCATTCTCC TGCTGCACTA AAGATCCCGC AGAAGATGTC ATCAACACTC 661 AGTGTGGCTA TGATGCCAGG CAAAAACCAG AAGTTGACCA GCAGATTGTA ATCTACACGA 721 AAGGCTGTGT GCCCCAGTTT GAGAAGTGGT TGCAGGACAA TTTAACCATC GTTGCTGGTA 781 TTTTCATAGG CATTGCATTG CTGCAGATAT TTGGGATATG CCTGGCCCAG AATTTGGTTA 841 GCGATATCGA AGCTGTCAGG GCGAGCTGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 901 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 961 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGTG 1021 CATACCCAGC ACTGCTTTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1081 tgaaagatt