Construct: ORF TRCN0000468722

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010101.1_s317c1
Derived from:
ccsbBroadEn_00758
DNA Barcode:
GACAACGTCTGGAAGTTCCATGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HMGA1 (3159)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468722
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319078.2 100% 100%
2 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319079.2 100% 100%
3 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319081.2 100% 100%
4 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319082.2 100% 100%
5 human 3159 HMGA1 high mobility group AT-hook 1 NM_145899.3 100% 100%
6 human 3159 HMGA1 high mobility group AT-hook 1 NM_145901.2 100% 100%
7 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319080.2 99% 99% 133_134insAGA
8 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319077.2 89.7% 89.7% 101_102ins33
9 human 3159 HMGA1 high mobility group AT-hook 1 NM_002131.4 89.7% 89.7% 101_102ins33
10 human 3159 HMGA1 high mobility group AT-hook 1 NM_145902.3 89.7% 89.7% 101_102ins33
11 human 3159 HMGA1 high mobility group AT-hook 1 NM_145903.3 89.7% 89.7% 101_102ins33
12 human 3159 HMGA1 high mobility group AT-hook 1 NM_145905.2 89.7% 89.7% 101_102ins33
13 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001025427.3 91.9% 97.1% (many diffs)
14 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001039356.2 91.9% 97.1% (many diffs)
15 mouse 111241 Hmga1-rs1 high mobility group AT-hook... NM_001166476.1 91.9% 97.1% (many diffs)
16 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166535.1 91.9% 97.1% (many diffs)
17 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166536.1 91.9% 97.1% (many diffs)
18 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_016660.3 91.9% 97.1% (many diffs)
19 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166543.1 90.9% 96.2% (many diffs)
20 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166544.1 90.9% 96.2% (many diffs)
21 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166545.1 88.5% 93.6% (many diffs)
22 mouse 111241 Hmga1-rs1 high mobility group AT-hook... NM_001166477.1 82.5% 86.9% (many diffs)
23 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166537.1 82.5% 86.9% (many diffs)
24 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166539.1 82.5% 86.9% (many diffs)
25 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166540.1 82.5% 86.9% (many diffs)
26 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166541.1 82.5% 86.9% (many diffs)
27 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166542.1 82.5% 86.9% (many diffs)
28 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166546.1 81.5% 66.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
387
ORF length:
321
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag tgagtcgagc tcgaagtcca gccagccctt ggcctccaag caggaaaagg
121acggcactga gaagcggggc cggggcaggc cgcgcaagca gccTCCGGTG AGTCCCGGGA
181CAGCGCTGGT AGGGAGTCAG AAGGAGCCCA GCGAAGTGCC AACACCTAAG AGACCTCGGG
241GCCGACCAAA GGGAAGCAAA AACAAGGGTG CTGCCAAGAC CCGGAAAACC ACCACAACTC
301CAGGAAGGAA ACCAAGGGGC AGACCCAAAA AACTGGAGAA GGAGGAAGAG GAGGGCATCT
361CGCAGGAGTC CTCGGAGGAG GAGCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
421GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
481AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACAACGT
541CTGGAAGTTC CATGGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
601aagatt

Download FASTA (ORF) (Full)

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