Construct: ORF TRCN0000468723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002776.1_s317c1
- Derived from:
- ccsbBroadEn_11463
- DNA Barcode:
- CTTGAGGTAACTACCAGAGTAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERF2 (10169)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10169 | SERF2 | small EDRK-rich factor 2 | NM_001199876.1 | 73.6% | 66.1% | 1_82del;115_137del |
| 2 | human | 10169 | SERF2 | small EDRK-rich factor 2 | NM_001199875.1 | 57.6% | 52% | 1_82del;115_248del |
| 3 | human | 10169 | SERF2 | small EDRK-rich factor 2 | NM_001199878.2 | 28.4% | 7% | 1_40del;135_136ins199 |
| 4 | human | 10169 | SERF2 | small EDRK-rich factor 2 | NM_001018108.4 | 25.2% | 6.2% | 1_82del;177_178ins199 |
| 5 | human | 10169 | SERF2 | small EDRK-rich factor 2 | NM_001199877.2 | 25.2% | 6.2% | 1_82del;177_178ins199 |
| 6 | human | 10169 | SERF2 | small EDRK-rich factor 2 | NR_037672.1 | 11.5% | (many diffs) | |
| 7 | mouse | 378702 | Serf2 | small EDRK-rich factor 2 | NM_001290837.1 | 71.4% | 61.7% | (many diffs) |
| 8 | mouse | 378702 | Serf2 | small EDRK-rich factor 2 | NM_011354.3 | 25% | 6.2% | 1_82del;87T>C;177_178ins199 |
| 9 | mouse | 378702 | Serf2 | small EDRK-rich factor 2 | NR_110986.1 | 8.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 360
- ORF length:
- 294
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gggctttcTG CTGCCGCCCG CAAGCAGAGG GACTCGGAGA TCATGCAGCA 121 GAAGCAGAAA AAGGCAAACG AGAAGAAGGA GGAACCCAAG TAGCTTTGTG GCTTCGTGTC 181 CAACCCTCTT GCCCTTCGCC TGTGTGCCTG GAGCCAGTCC CACCACGCTC GCGTTTCCTC 241 CTGTAGTGCT CACAGGTCCC AGCACCGATG GCATTCCCTT TGCCCTGAGT CTGCAGCGGG 301 TCCCTTTTGT GCTTCCTTCC CCTCAGGTAG CCTCTCTCCC CCTGGGCCAC TCCCGGGGGT 361 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 481 TTTATATATC TTGTGGAAAG GACGACTTGA GGTAACTACC AGAGTAATGA CGCGTTAAGT 541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt