Construct: ORF TRCN0000468747
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002778.1_s317c1
- Derived from:
- ccsbBroadEn_08795
- DNA Barcode:
- ATAGACGGAGAAGCAGACGTCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPCDC (60490)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468747
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NM_021823.5 | 99.6% | 99% | 16T>A;234A>G |
| 2 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | XM_017022465.2 | 87.8% | 82.2% | (many diffs) |
| 3 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NM_001301102.2 | 83.9% | 83.3% | 16T>A;134_135ins96;138A>G |
| 4 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NM_001301101.2 | 78.7% | 78.4% | 16T>A;231_232ins129 |
| 5 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | XM_017022461.1 | 73.1% | 67.7% | (many diffs) |
| 6 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NM_001301103.2 | 63% | 62.7% | 16T>A;135_136ins225 |
| 7 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | XM_017022462.1 | 59.4% | 46.1% | (many diffs) |
| 8 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | XM_017022464.1 | 53.6% | 48.6% | (many diffs) |
| 9 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NM_001301104.2 | 39.7% | 39.7% | 0_1ins369 |
| 10 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NM_001301105.2 | 39.7% | 39.7% | 0_1ins369 |
| 11 | human | 60490 | PPCDC | phosphopantothenoylcysteine... | NR_125369.2 | 23.7% | (many diffs) | |
| 12 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | NM_176831.4 | 86.1% | 87.7% | (many diffs) |
| 13 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_006511360.3 | 86.1% | 87.7% | (many diffs) |
| 14 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_006511361.3 | 86.1% | 87.7% | (many diffs) |
| 15 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_006511362.3 | 86.1% | 87.7% | (many diffs) |
| 16 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_006511363.3 | 86.1% | 87.7% | (many diffs) |
| 17 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_011242789.2 | 86.1% | 87.7% | (many diffs) |
| 18 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_017313518.1 | 86.1% | 87.7% | (many diffs) |
| 19 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XM_006511367.3 | 54.2% | 53.9% | (many diffs) |
| 20 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XR_379440.3 | 23.7% | (many diffs) | |
| 21 | mouse | 66812 | Ppcdc | phosphopantothenoylcysteine... | XR_379441.3 | 19.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga accaaaggcc acctgtccag ctgctgcacc cttgatggag agaaaattcc 121 atgttcttgt gggtgtcacg gggagtgtcg cagccctgaa gttgcctctt ctggtgtcaa 181 agcttttgga cattcctggg ctggaagtag cagtggTCAC AACTGAGAGA GCCAAACATT 241 TCTACAGCCC CCAGGACATT CCTGTCACCC TCTACAGCGA CGCTGATGAA TGGGAGATGT 301 GGAAGAGCCG CTCTGACCCA GTTCTGCACA TTGACCTGCG GAGGTGGGCA GACCTCCTGC 361 TGGTGGCTCC TCTTGATGCC AACACTCTGG GGAAGGTGGC CAGTGGCATC TGTGACAACT 421 TGCTTACCTG CGTCATGCGG GCCTGGGACC GCAGCAAGCC CCTGCTCTTC TGCCCGGCCA 481 TGAACACCGC CATGTGGGAG CACCCGATCA CAGCGCAGCA GGTAGACCAG CTCAAGGCCT 541 TTGGCTATGT CGAGATCCCC TGTGTGGCCA AGAAGCTGGT GTGCGGAGAT GAAGGTCTCG 601 GGGCCATGGC TGAAGTGGGG ACCATCGTGG ACAAAGTGAA AGAAGTCCTC TTCCAGCACA 661 GTGGCTTCCA GCAGAGTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATAGACG GAGAAGCAGA 841 CGTCGGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt