Construct: ORF TRCN0000468748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004182.1_s317c1
- Derived from:
- ccsbBroadEn_03027
- DNA Barcode:
- ATTTCAACAACACGAGCGACACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB30 (27314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_001286059.2 | 100% | 100% | |
| 2 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_001286060.2 | 100% | 100% | |
| 3 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_001286061.1 | 100% | 100% | |
| 4 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_014488.5 | 100% | 100% | |
| 5 | mouse | 75985 | Rab30 | RAB30, member RAS oncogene ... | NM_029494.2 | 94% | 100% | (many diffs) |
| 6 | mouse | 75985 | Rab30 | RAB30, member RAS oncogene ... | XM_006508293.3 | 94% | 100% | (many diffs) |
| 7 | mouse | 75985 | Rab30 | RAB30, member RAS oncogene ... | XM_006508294.2 | 94% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 675
- ORF length:
- 609
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tatggaagat tatgatttcc tgttcaaaat tgttttaatt ggcaacgctg 121 gtgtggggaa gacgtgcctc gtccgaagat tcactcaggg tcttttcccc ccaggTCAAG 181 GAGCCACAAT TGGAGTTGAT TTTATGATTA AGACAGTGGA GATTAATGGT GAAAAAGTAA 241 AGCTACAGAT CTGGGACACA GCAGGTCAAG AGAGATTTCG GTCCATTACC CAGAGTTACT 301 ACCGAAGCGC CAATGCCTTG ATCCTCACCT ATGACATTAC CTGTGAGGAA TCCTTCCGTT 361 GCCTTCCTGA GTGGCTGCGG GAGATAGAAC AATATGCCAG CAACAAGGTC ATCACTGTGT 421 TAGTGGGCAA CAAGATTGAC CTGGCTGAAA GGAGAGAGGT TTCCCAGCAG CGAGCTGAAG 481 AATTCTCAGA AGCTCAGGAC ATGTATTATC TGGAGACCTC AGCCAAGGAA TCTGATAATG 541 TGGAGAAACT CTTCCTTGAC TTAGCATGCC GACTCATCAG TGAAGCCAGA CAGAACACAC 601 TTGTGAACAA TGTATCCTCA CCCTTACCTG GAGAAGGGAA AAGCATCAGC TATTTGACTT 661 GTTGTAATTT CAACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 721 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 781 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTTCAACAA CACGAGCGAC 841 ACATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt