Construct: ORF TRCN0000468751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011516.1_s317c1
- Derived from:
- ccsbBroadEn_00490
- DNA Barcode:
- TGAACACGATACCGGTTTCGATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4EBP1 (1978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1978 | EIF4EBP1 | eukaryotic translation init... | NM_004095.4 | 100% | 100% | |
| 2 | mouse | 13685 | Eif4ebp1 | eukaryotic translation init... | NM_007918.3 | 89.8% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 444
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCCGGGGG CAGCAGCTGC AGCCAGACCC 121 CAAGCCGGGC CATCCCCGCC ACTCGCCGGG TGGTGCTCGG CGACGGCGTG CAGCTCCCGC 181 CCGGGGACTA CAGCACGACC CCCGGCGGCA CGCTCTTCAG CACCACCCCG GGAGGTACCA 241 GGATCATCTA TGACCGGAAA TTCCTGATGG AGTGTCGGAA CTCACCTGTG ACCAAAACAC 301 CCCCAAGGGA TCTGCCCACC ATTCCGGGGG TCACCAGCCC TTCCAGTGAT GAGCCCCCCA 361 TGGAAGCCAG CCAGAGCCAC CTGCGCAATA GCCCAGAAGA TAAGCGGGCG GGCGGTGAAG 421 AGTCACAGTT TGAGATGGAC ATTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GAACACGATA 601 CCGGTTTCGA TCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att