Construct: ORF TRCN0000468759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001029.1_s317c1
- Derived from:
- ccsbBroadEn_06462
- DNA Barcode:
- CCTAGGCAGGACCTTTAACCAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGAE (3682)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3682 | ITGAE | integrin subunit alpha E | NM_002208.5 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 3682 | ITGAE | integrin subunit alpha E | XM_024450740.1 | 96.7% | 96.6% | (many diffs) |
| 3 | human | 3682 | ITGAE | integrin subunit alpha E | XM_011523827.3 | 95.7% | 95.5% | (many diffs) |
| 4 | human | 3682 | ITGAE | integrin subunit alpha E | XM_017024586.1 | 94.6% | 94.4% | (many diffs) |
| 5 | human | 3682 | ITGAE | integrin subunit alpha E | XM_011523825.3 | 94.1% | 94% | (many diffs) |
| 6 | human | 3682 | ITGAE | integrin subunit alpha E | XM_011523828.3 | 93.5% | 93.3% | (many diffs) |
| 7 | human | 3682 | ITGAE | integrin subunit alpha E | XM_017024587.1 | 69.3% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3606
- ORF length:
- 3537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggctcttc cacactctgc tctgcatagc cagcctggcc ctgctggccg 121 ctttcaatgt ggatgtggcc cggccctggc tcacgcccaa gggaggtgcc cctttcgtgc 181 tcagctccct tctgcaccaa gaccccagca ccaaccagac ctggctcctg gtcaccagcc 241 ccagaaccaa gaggacacca gggcccctcc atcgatgttc ccttgtccag gatgaaatcc 301 tttgccatcc tgtagagcat gtccccatcc ccaaggggag gcaccgggga gtgaccgttg 361 tccggagcca ccacggtgtt ttgatatgca ttcaagtgct ggtccggcgg cctcacagcc 421 tcagctcaga actcacaggc acctgtagcc tcctgggccc tgacctccgt ccccaggctc 481 aggccaactt cttcgacctt gaaaatctcc tggatccaga tgcacgtgtg gacactggag 541 actgctacag caacaaagaa ggcggtggag aagacgatgt gaacacagcc aggcagcgcc 601 gggctctgga gaaggaggag gaggaagaca aggaggagga ggaagacgag gaggaggagg 661 aagctggcac cgagattgcc atcatcctgg atggctcagg aagcattgat cccccagact 721 ttcagagagc caaagacttc atctccaaca tgatgaggaa cttctatgaa aagtgttttg 781 agtgcaactt tgccttggtg cagtatggag gagtgatcca gactgagttt gaccttcggg 841 acagccagga tgtgatggcc tccctcgcca gagtccagaa catcactcaa gtggggagtg 901 tcaccaagac tgcctcagcc atgcaacacg tcttagacag catcttcacc tcaagccacg 961 gctccaggag aaaggcatcc aaggtcatgg tggtgctcac cgatggtggc atattcgagg 1021 accccctcaa ccttacgaca gtcatcaact cccccaaaat gcagggtgtt gagcgctttg 1081 ccattggggt gggagaagaa tttaagagtg ctaggactgc gagggaactg aacctgatcg 1141 cctcagaccc ggatgagacc catgctttca aggtgaccaa ctacatggcg ctggatgggc 1201 tgctgagcaa actgcggtac aacatcatca gcatggaagg cacggttgga gacgcccttc 1261 actaccagct ggcacagatt ggcttcagtg ctcagatcct ggatgagcgg caggtgctgc 1321 tcggcgccgt cggggccttt gactggtccg gaggggcgtt gctctacgac acacgcagcc 1381 gccggggccg cttcctgaac cagacagcgg cggcggcggc agacgcggag gctgcgcagt 1441 acagctacct gggttacgct gtggccgtgc tgcacaagac ctgcagcctc tcctacgtcg 1501 cgggggctcc acagtacaaa catcatgggg ccgtgtttga gctccagaag gagggcagag 1561 aggccagctt cctgccagtg ctggagggag agcagatggg gtcctatttt ggctctgagc 1621 tgtgccctgt ggacattgac atggatggaa gcacggactt cttgctggtg gctgctccat 1681 tttaccacgt tcatggagaa gaaggcagag tctacgtgta ccgtctcagc gagcaggatg 1741 gttctttctc cttggcacgc atactgagtg ggcaccccgg gttcaccaat gcccgctttg 1801 gctttgccat ggcggctatg ggggatctca gtcaggataa gctcacagat gtggccatcg 1861 gggcccccct ggaaggtttt ggggcagatg atggtgccag cttcggcagt gtgtatatct 1921 acaatggaca ctgggacggc ctctccgcca gcccctcgca gcggatcaga gcctccacgg 1981 tggccccagg actccagtac ttcggcatgt ccatggctgg tggctttgat attagtggcg 2041 acggccttgc cgacatcacc gtgggcactc tgggccaggc ggttgtgttc cgctcccggc 2101 ctgtggttcg cctgaaggtc tccatggcct tcacccccag cgcactgccc atcggcttca 2161 acggcgtcgt gaatgtccgt ttatgttttg aaatcagctc tgtaaccaca gcctctgagt 2221 caggcctccg tgaggcactt ctcaacttca cgctggatgt ggatgtgggg aagcagagga 2281 gacggctgca gtgttcagac gtaagaagct gtctgggctg cctgagggag tggagcagcg 2341 gatcccagct ttgtgaggac ctcctgctca tgcccacaga gggagagctc tgtgaggagg 2401 actgcttctc caatgccagt gtcaaagtca gctaccagct ccagacccct gagggacaga 2461 cggaccatcc ccagcccatc ctggaccgct acactgagcc ctttgccatc ttccagctgc 2521 cctatgagaa ggcctgcaag aataagctgt tttgtgtcgc agaattacag ttggccacca 2581 ccgtctctca gcaggagttg gtggtgggtc tcacaaagga gctgaccctg aacattaacc 2641 taactaactc cggggaagat tcctacatga caagcatggc cttgaattac cccagaaacc 2701 tgcagttgaa gaggatgcaa aagcctccct ctccaaacat tcagtgtgat gaccctcagc 2761 cggttgcttc tgtcctgatc atgaactgca ggattggtca ccccgtcctc aagaggtcat 2821 ctgctcatgt ttcagtcgtt tggcagctag aggagaatgc ctttccaaac aggacagcag 2881 acatcactgt gactgtcacc aattccaatg aaagacggtc tttggccaac gagacccaca 2941 cccttcaatt caggcatggc ttcgttgcag ttctgtccaa accatccata atgtacgtga 3001 acacaggcca ggggctttct caccacaaag aattcctctt ccatgtacat ggggagaacc 3061 tctttggagc agaataccag ttgcaaattt gcgtcccaac caaattacga ggtctccagg 3121 ttgcagcagt gaagaagctg acgaggactc aggcctccac ggtgtgcacc tGGAGTCAGG 3181 AGCGCGCTTG TGCGTACAGT TCGGTTCAGC ATGTGGAAGA ATGGCATTCA GTGAGCTGTG 3241 TCATCGCTTC AGATAAAGAA AATGTCACCG TGGCTGCAGA GATCTCCTGG GATCACTCTG 3301 AGGAGTTACT AAAAGATGTA ACTGAACTGC AGATCCTTGG TGAAATATCT TTCAACAAAT 3361 CTCTATATGA GGGACTGAAT GCAGAGAACC ACAGAACTAA GATCACTGTC GTCTTCCTGA 3421 AAGATGAGAA GTACCATTCT TTGCCTATCA TCATTAAAGG CAGCGTTGGT GGACTTCTGG 3481 TGTTGATCGT GATTCTGGTC ATCCTGTTCA AGTGTGGCTT TTTTAAAAGA AAATATCAAC 3541 AACTGAACTT GGAGAGCATC AGGAAGGCCC AGCTGAAATC AGAGAATCTG CTCGAAGAAG 3601 AGAATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3721 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTAGGCAG GACCTTTAAC CAAAAACGCG 3781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt