Construct: ORF TRCN0000468766
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000438.1_s317c1
- Derived from:
- ccsbBroadEn_04294
- DNA Barcode:
- TCTCAATACCCAATGCCCTTTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG10 (83734)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468766
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83734 | ATG10 | autophagy related 10 | NM_001131028.2 | 100% | 100% | |
| 2 | human | 83734 | ATG10 | autophagy related 10 | NM_031482.5 | 100% | 100% | |
| 3 | human | 83734 | ATG10 | autophagy related 10 | XM_005248610.5 | 100% | 100% | |
| 4 | human | 83734 | ATG10 | autophagy related 10 | XM_005248611.5 | 100% | 100% | |
| 5 | human | 83734 | ATG10 | autophagy related 10 | XM_005248612.3 | 83.6% | 83.6% | 106_107ins108 |
| 6 | human | 83734 | ATG10 | autophagy related 10 | XM_017009944.1 | 83.6% | 83.6% | 106_107ins108 |
| 7 | human | 83734 | ATG10 | autophagy related 10 | XM_011543660.2 | 80.9% | 80.9% | 0_1ins126 |
| 8 | human | 83734 | ATG10 | autophagy related 10 | XM_011543661.2 | 67.8% | 67.7% | 0_1ins210;4_5delCTinsGA |
| 9 | mouse | 66795 | Atg10 | autophagy related 10 | XM_011244551.2 | 70% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 726
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaagatgag ttcattggag aaaaaacatt ccaacgttat tgtgcagaat 121 tcattaaaca ttcacaacag ataggtgata gttgggaatg gagaccatca aaggactgtt 181 ctgatggcta catgtgcaaa atacactttc aaattaagaa tgggtctgtg atgtcacatc 241 taggagcatc tacccatgga cagacatgTC TTCCCATGGA GGAGGCTTTC GAGCTACCCT 301 TGGATGATTG TGAAGTGATT GAAACTGCAG CAGCGTCCGA AGTGATTAAA TATGAGTATC 361 ATGTCTTATA TTCCTGTAGC TACCAAGTGC CTGTACTTTA CTTTAGGGCA AGCTTTTTAG 421 ATGGGAGACC TTTAACTCTG AAGGACATAT GGGAAGGAGT TCATGAGTGC TATAAGATGC 481 GACTGCTACA GGGACCATGG GACACTATTA CGCAACAGGA ACATCCAATA CTTGGGCAAC 541 CCTTTTTTGT ACTTCATCCC TGCAAGACGA ATGAATTCAT GACTCCTGTA TTAAAGAATT 601 CTCAGAAAAT CAATAAGAAT GTCAACTATA TCACATCATG GCTGAGCATT GTAGGGCCAG 661 TTGTTGGGCT GAATCTACCT CTGAGTTATG CCAAAGCAAC GTCTCAGGAT GAACGAAATG 721 TCCCTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTCAATAC CCAATGCCCT TTTTGACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt