Construct: ORF TRCN0000468782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004827.1_s317c1
- Derived from:
- ccsbBroadEn_09881
- DNA Barcode:
- CCTCTGGACCCAAGGTCATGTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFX6 (222546)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222546 | RFX6 | regulatory factor X6 | NM_173560.4 | 99.9% | 99.7% | 16G>A;2062A>G |
| 2 | human | 222546 | RFX6 | regulatory factor X6 | XM_011535589.1 | 96% | 95.9% | 16G>A;670_671ins108;1954A>G |
| 3 | human | 222546 | RFX6 | regulatory factor X6 | XM_017010477.1 | 86.3% | 86.2% | 1_2delATins380;1684A>G |
| 4 | mouse | 320995 | Rfx6 | regulatory factor X, 6 | NM_001159389.1 | 85.9% | 87.1% | (many diffs) |
| 5 | mouse | 320995 | Rfx6 | regulatory factor X, 6 | NM_177306.3 | 61.5% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2850
- ORF length:
- 2784
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaggtcccg aagctggaag acaccttcct gcaggcgcag cctgcgcccc 121 aactgtcccc ggggatccag gaagactgct gtgtgcagct cctgggcaag ggcttgctag 181 tctatccgga agaaacagtg tacctggcgg ccgaagggca gcccgggggc gagcagggcg 241 gcggggagaa aggcgaagac ccggagctgc cgggggcagt gaaatcagaa atgcacttaa 301 acaatggtaa cttttcctct gaagaagagg acgccgacaa ccacgacagc aaaaccaaag 361 cagcggatca atacctgtct cagaagaaaa ccatcacgca gattgtgaag gataaaaaga 421 agcagacaca gctcacgctg cagtggcttg aagagaatta cattgtatgt gaaggagttt 481 gcttaccacg gtgcattctt tatgcacact acttagattt ctgtaggaaa gagaaattag 541 agccagcctg tgcggccacc tttggaaaga caattcgcca gaagtttccc ctcctaacaa 601 caaggcggct tggaacaaga ggccattcaa agtatcatta ctatgggatt ggcatcaaag 661 agagcagtgc atattaccac tccgtttatt ctggaaaggg cttgacaagg ttttctggaa 721 gcaagctaaa gaatgagggt ggcttcactc gtaaatattc gcttagctca aaaactggaa 781 cacttcttcc agaattcccc agcgctcaac accttgtata ccaaggatgc atttctaagg 841 acaaggttga tacgctcata atgatgtaca aaactcactg ccagtgtatc ctggacaatg 901 caattaatgg aaactttgaa gagatccagc attttttatt acacttttgg caaggaatgc 961 ctgaccatct ccttcccctg ctcgaaaatc ctgttatcat tgatattttc tgtgtttgtg 1021 actcaattct ttataaggtt cttacagatg tactcattcc tgcaacaatg caagaaatgc 1081 ctgaaagctt attagcagac ataagaaatt ttgctaaaaa ttgggaacag tgggttgttt 1141 catccttgga aaacttgcca gaagctctaa ctgacaagaa aatacctatt gtgcgaagat 1201 ttgtatcttc tctgaaacga caaacatctt tcttacatct tgcccagatt gccagaccag 1261 ctctctttga ccagcatgtc gttaattcta tggtgtctga tattgaaagg gttgatttga 1321 acagcattgg ctctcaagcc cttcttacca tttcaggcag cacagacact gaatctggta 1381 tctacactga acatgactct atcactgtgt tccaagaact gaaggatctc cttaagaaga 1441 atgccactgt ggaggctttt attgaatggt tggatactgt ggtagaacag agagttatta 1501 agaccagcaa acaaaatgga aggtcattaa agaagagagc tcaagacttt ctgttaaagt 1561 ggagtttttt tggtgctcga gtaatgcata atctcacctt gaacaatgca tccagttttg 1621 gttcttttca tttgattcga atgcttctcg atgaatacat tctcctggcc atggagaccc 1681 agtttaataa tgacaaagag caggagttac agaatttatt ggacaagtat atgaagaatt 1741 cagatgcgag taaagctgct ttcactgctt ctccgagttc atgctttctg gccaaccgta 1801 ataaagggag catggtttcc agcgacgctg tgaagaatga aagccacgtg gagacaacct 1861 atctccctct gccatccagt caacctggag gcctaggccc tgctctgcac cagttccctg 1921 ctgggaacac agacaacatg ccgctcacag gtcaaatgga gctttcacag attgctggtc 1981 atctgatgac accacccatt tctccagcca tggcaagccg aggaagtgtc attaaccaag 2041 gaccaatggc agggaggccc ccaagtgtgg gcccagtact gtcagctcca tcacactgct 2101 ccacataccc agagcccatt tatcccgctc tccctcaagc caatcatgac ttttatagca 2161 ccagctctaa ctaccagact gtgtttaggg cacagcccca ctccacatca ggactctatc 2221 ctcatcacac cgagcatggt cgatgcatgg cttggactga acagcagctt tcaagagact 2281 tcttcagtgg cagctgtgcg gggtctccat ataactcccg gccaccgtct agctatggcc 2341 catccctgca agcccaggat tcacacaata tgcagttttt aaatacagga agcttcaatt 2401 tcttgagcaa cacaggagct gccagctgcc aaggagcaac actgcctcct aattcaccaa 2461 atggatacta tggaagcaac ataaactacc cagagtctca caggctcgga tcaatggtga 2521 atcagcacgt ttctgtcatc agcagcattc gttcactgcc cccctacagt gacatccacg 2581 atccacttaa cattttagat gacagtggta gaaaacagac cagctcgttt tacacagaca 2641 catcatctcc agttgcatgt cgaactccag tcctagcttc cagtttgcaa accccaattc 2701 cttcttcctc atcccaatgt atgtatggaa cttccaacca gtatccagct caagaaaccc 2761 tggactccca tggaacaagc agtagagaaa tggtgtcctc tttaccacct atcaacactg 2821 tgttcatggg aacagcagct GGAGGCACTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2881 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2941 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTCT 3001 GGACCCAAGG TCATGTAGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3061 tgaaagatt