Construct: ORF TRCN0000468792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014906.1_s317c1
- Derived from:
- ccsbBroadEn_01063
- DNA Barcode:
- ACTTGCCCCCGTTTATGTCCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDP (4693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4693 | NDP | norrin cystine knot growth ... | NM_000266.4 | 100% | 100% | |
| 2 | mouse | 17986 | Ndp | Norrie disease (pseudogliom... | NM_010883.3 | 90.4% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 489
- ORF length:
- 399
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGAAAACA TGTACTAGCT GCATCCTTTT 121 CTATGCTCTC CCTGCTGGTG ATAATGGGAG ATACAGACAG TAAAACGGAC AGCTCATTCA 181 TAATGGACTC GGACCCTCGA CGCTGCATGA GGCACCACTA TGTGGATTCT ATCAGTCACC 241 CATTGTACAA GTGTAGCTCA AAGATGGTGC TCCTGGCCAG GTGCGAGGGG CACTGCAGCC 301 AGGCGTCACG CTCCGAGCCT TTGGTGTCGT TCAGCACTGT CCTCAAGCAA CCCTTCCGTT 361 CCTCCTGTCA CTGCTGCCGG CCCCAGACTT CCAAGCTGAA GGCACTGCGG CTGCGATGCT 421 CAGGGGGCAT GCGACTCACT GCCACCTACC GGTACATCCT CTCCTGTCAC TGCGAGGAAT 481 GCAATTCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTTGC CCCCGTTTAT GTCCGTTCAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt