Construct: ORF TRCN0000468794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014765.1_s317c1
- Derived from:
- ccsbBroadEn_04609
- DNA Barcode:
- AAGTTTACCTAGTGCCACTCTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5IF1 (93974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_016311.5 | 100% | 100% | |
| 2 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_178190.3 | 63.2% | 61.3% | (many diffs) |
| 3 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_178191.2 | 56.2% | 56.6% | 180_180delGins139 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 408
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCAGTGAC GGCGTTGGCG GCGCGGACGT 121 GGCTTGGCGT GTGGGGCGTG AGGACCATGC AAGCCCGAGG CTTCGGCTCG GATCAGTCCG 181 AGAATGTCGA CCGGGGCGCG GGCTCCATCC GGGAAGCCGG TGGGGCCTTC GGAAAGAGAG 241 AGCAGGCTGA AGAGGAACGA TATTTCCGAG CACAGAGTAG AGAACAACTG GCAGCTTTGA 301 AAAAACACCA TGAAGAAGAA ATCGTTCATC ATAAGAAGGA GATTGAGCGT CTGCAGAAAG 361 AAATTGAGCG CCATAAGCAG AAGATCAAAA TGCTAAAACA TGATGATTAC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAAAGTTTA CCTAGTGCCA CTCTCTAACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt