Construct: ORF TRCN0000468803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002742.1_s317c1
- Derived from:
- ccsbBroadEn_15493
- DNA Barcode:
- AGTAGGTACAACATGCGTTGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAS1 (4142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4142 | MAS1 | MAS1 proto-oncogene, G prot... | NM_001366704.1 | 99.8% | 100% | 633C>T |
| 2 | human | 4142 | MAS1 | MAS1 proto-oncogene, G prot... | NM_002377.4 | 99.8% | 100% | 633C>T |
| 3 | mouse | 17171 | Mas1 | MAS1 oncogene | NM_008552.5 | 85.3% | 90.1% | (many diffs) |
| 4 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_006523293.4 | 85.3% | 90.1% | (many diffs) |
| 5 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_006523294.4 | 85.3% | 90.1% | (many diffs) |
| 6 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_006523296.4 | 85.3% | 90.1% | (many diffs) |
| 7 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246190.3 | 85.3% | 90.1% | (many diffs) |
| 8 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246191.3 | 85.3% | 90.1% | (many diffs) |
| 9 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246192.3 | 85.3% | 90.1% | (many diffs) |
| 10 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_011246193.3 | 85.3% | 90.1% | (many diffs) |
| 11 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_017317270.2 | 85.3% | 90.1% | (many diffs) |
| 12 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_030249523.1 | 85.3% | 90.1% | (many diffs) |
| 13 | mouse | 17171 | Mas1 | MAS1 oncogene | XM_030249524.1 | 85.3% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1044
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgggtca aacgtgacat catttgttgt tgaggaaccc acgaacatct 121 caactggcag gaacgcctca gtcgggaatg cacatcggca aatccccatc gtgcactggg 181 tcattatgag catctcccca gtggggtttg ttgagaatgg gattctcctc tggttcctgt 241 gcttccggat gagaagaaat cccttcactg tctacatcac ccacctgtct atcgcagaca 301 tctcactgct cttctgtatt ttcatcttgt ctatcgacta tgctttagat tatgagcttt 361 cttctggcca ttactacaca attgtcacat tatcagtgac ttttctgttt ggctacaaca 421 cgggcctcta tctgctgacg gccattagtg tggagaggtg cctgtcagtc ctttacccca 481 tctggtaccg atgccatcgc cccaagtacc agtcggcatt ggtctgtgcc cttctgtggg 541 ctctttcttg cttggtgacc accatggagt atgtcatgtg catcgacaga gaagaagaga 601 gtcactctcg gaatgactgc cgagcagtca tcatctttat agccatccTG AGCTTCCTGG 661 TCTTCACGCC CCTCATGCTG GTGTCCAGCA CCATCTTGGT TGTGAAGATC CGGAAGAACA 721 CGTGGGCTTC CCATTCCTCC AAGCTTTACA TAGTCATCAT GGTCACCATC ATTATATTCC 781 TCATCTTCGC TATGCCCATG AGACTCCTTT ACCTGCTGTA CTATGAGTAT TGGTCGACCT 841 TTGGGAACCT ACACCACATT TCCCTGCTCT TCTCCACAAT CAACAGTAGC GCCAACCCTT 901 TCATTTACTT CTTTGTGGGA AGCAGTAAGA AGAAGAGATT CAAGGAGTCC TTAAAAGTTG 961 TTCTGACCAG GGCTTTCAAA GATGAAATGC AACCTCGGCG CCAGAAAGAC AATTGTAATA 1021 CGGTCACAGT TGAGACTGTC GTCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GTAGGTACAA 1201 CATGCGTTGT ATCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att