Construct: ORF TRCN0000468819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014808.1_s317c1
- Derived from:
- ccsbBroadEn_00496
- DNA Barcode:
- TAACAAAACTGAGGTAAACTTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELF4 (2000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2000 | ELF4 | E74 like ETS transcription ... | NM_001127197.2 | 100% | 100% | |
| 2 | human | 2000 | ELF4 | E74 like ETS transcription ... | NM_001421.3 | 100% | 100% | |
| 3 | human | 2000 | ELF4 | E74 like ETS transcription ... | XM_005262389.3 | 100% | 100% | |
| 4 | human | 2000 | ELF4 | E74 like ETS transcription ... | XM_011531307.3 | 89.7% | 89.7% | 0_1ins204 |
| 5 | human | 2000 | ELF4 | E74 like ETS transcription ... | XM_011531308.3 | 88.2% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2055
- ORF length:
- 1989
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tattacccta cagcccagtg acctgatctt tgagttcgca agcaacggga 121 tggatgatga tatccaccag ctggaagacc cctctgtgtt cccagctgtg atcgtggagc 181 aggtacccta ccctgattta ctgcatctgt actcgggact ggagttggac gacgttcaca 241 atggcatcat aacagacggg accttgtgca tgacgcagga tcagatcctg gaaggcagtt 301 ttttgctgac agatgacaat gaggccacct cgcacaccat gtcaaccgcg gaagtcttac 361 tcaatatgga gtctcccagc gatatcctgg atgagaagca gatcttcagt acctccgaaa 421 tgcttccaga ctcggaccct gcaccagctg tcactctgcc caactacctg tttcctgcct 481 ctgagcccga tgccctgaac agggcgggtg acactagtga ccaggagggg cattctctgg 541 aggagaaggc ctccagagag gaaagtgcca agaagactgg gaaatcaaag aagagaatcc 601 ggaagaccaa gggcaaccga agtacctcac ctgtcactga ccccagcatc cccattagga 661 agaaatcaaa ggatggcaaa ggcagcacca tctatctgtg ggagttcctc ctggctcttc 721 tgcaagacag aaacacctgt cccaagtaca tcaagtggac ccagcgagag aaaggcatct 781 tcaaactggt ggactccaaa gctgtgtcca agctgtgggg gaagcagaaa aacaagcctg 841 acatgaacta tgagacaatg gggcgggcac taagatacta ctaccaaaga ggcatactgg 901 ccaaagtgga agggcagagg ctggtgtacc agtttaagga gatgcccaag gacctggtgg 961 tcattgaaga tgaggatgag agcagcgaag ccacagcagc cccacctcag gcctccacgg 1021 cctctgtggc ctctgccagt accacccggc gaaccagctc cagggtctca tccagatctg 1081 ccccccaggg caagggcagc tcttcttggg agaagccaaa aattcagcat gtcggtctcc 1141 agccatctgc gagtctggaa ttgggaccgt cgctagacga ggagatcccc actacctcca 1201 ccatgctcgt ctctccagca gagggccagg tcaagctcac caaagctgtg agtgcatctt 1261 cagtgcccag caacatccac ctaggagtgg cccccgtggg gtcgggctcg gccctgaccc 1321 tgcagacgat cccactgacc acggtgctga ccaatgggcc tcctgccagt actactgctc 1381 ccactcagct cgttctccag agtgttccag cggcctctac tttcaaggac accttcactt 1441 tgcaggcctc tttccccctg aacgccagtt tccaagacag ccaggtggca gccccagggg 1501 ctccactgat tctcagtggc ctcccccaac ttctggctgg ggccaaccgt ccgaccaacc 1561 cggcgccacc cacggtcaca ggggctggac cagcagggcc cagctctcag ccccctggga 1621 ctgtcattgc tgccttcatc aggacttctg gcactacagc agcccctagg gtcaaggagg 1681 ggccactgag gtcctcctcc tatgttcagg gtatggtgac gggggccccc atggaggggc 1741 tgctggttcc tgaagagacc ctgagggagc tcctgagaga tcaggctcat cttcagccac 1801 ttccaaccca ggtggtttcc aggggttccc acaatccgag ccttctgggc aaccagactt 1861 tgtctcctcc cagccgcccc actgttgggc tgaccccagt ggctgaactt gagctctcct 1921 caggctcagg gtccctgctg atggctgagc ctagtgtgac cacatctggg agccttctGA 1981 CAAGATCCCC CACCCCAGCC CCTTTCTCCC CATTCAACCC TACTTCCCTC ATTAAGATGG 2041 AGCCCCATGA CATATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2101 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2161 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAACAAAACT GAGGTAAACT 2221 TTACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt