Construct: ORF TRCN0000468823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014046.1_s317c1
- Derived from:
- ccsbBroadEn_04205
- DNA Barcode:
- AGTCCTTGTGGCCTGCATTCGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL15 (80311)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80311 | KLHL15 | kelch like family member 15 | NM_030624.3 | 100% | 100% | |
| 2 | mouse | 236904 | Klhl15 | kelch-like 15 | NM_001039061.1 | 90.8% | 99.3% | (many diffs) |
| 3 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_006527986.3 | 90.8% | 99.3% | (many diffs) |
| 4 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_006527987.2 | 90.8% | 99.3% | (many diffs) |
| 5 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_006527988.3 | 90.8% | 99.3% | (many diffs) |
| 6 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_006527989.3 | 90.8% | 99.3% | (many diffs) |
| 7 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_006527990.3 | 90.8% | 99.3% | (many diffs) |
| 8 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_011247581.1 | 90.8% | 99.3% | (many diffs) |
| 9 | mouse | 236904 | Klhl15 | kelch-like 15 | NM_001039060.1 | 37.1% | 39.2% | (many diffs) |
| 10 | mouse | 236904 | Klhl15 | kelch-like 15 | NM_001039059.1 | 35.8% | 38.5% | (many diffs) |
| 11 | mouse | 236904 | Klhl15 | kelch-like 15 | NM_153165.2 | 35.8% | 38.5% | (many diffs) |
| 12 | mouse | 236904 | Klhl15 | kelch-like 15 | XM_006527991.3 | 35.8% | 38.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1881
- ORF length:
- 1812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaggggac gtggaaggat tctgttcctc catccacgac accagtgtct 121 ctgctgggtt cagagcactg tatgaggagg gattgcttct tgatgtcact ctggttattg 181 aagatcatca gttccaggcc cataaagcac tcttggccac ccagagtgat tacttcagaa 241 ttatgtttac tgcagacatg agggaacgag atcaggacaa aattcattta aaaggtctaa 301 cagctaccgg tttcagccat gtcctgcaat ttatgtacta tggaactata gagctgagta 361 tgaataccgt tcatgagatt cttcaggctg ccatgtatgt tcaacttata gaagtggtga 421 agttctgctg ctcttttctc ttagcgaaga tctgcctaga aaattgtgca gaaattatga 481 gactcttaga tgatttcggc gtaaacatcg agggagtcag ggagaagtta gacacctttc 541 tgctagacaa ctttgtgcca ctcatgtcta ggcctgactt tctgtcctat ctgagctttg 601 agaagctcat gtcttacttg gataatgatc atctgagcag gttcccagag atagagctgt 661 acgaggctgt gcagtcttgg ctgcggcatg atagaagacg ctggagacat accgatacca 721 tcattcagaa tatccggttt tgcttgatga ccccaaccag cgtttttgag aaggttaaga 781 catcagaatt ttatagatac tcccgacagc tccgttacga agttgaccaa gcattgaatt 841 actttcagaa tgttcaccag cagcctttgt tggatatgaa gtcaagccgc atccgttctg 901 caaaaccgca aactacagta tttcgaggaa tgattggaca tagcatggtt aacagtaaaa 961 tacttctctt aaagaaacca agagtctggt gggagctaga aggcccacaa gtacctctgc 1021 gacctgactg ccttgctatc gtcaataatt ttgtgttcct gttaggcggg gaagagctgg 1081 gcccggatgg tgaattccat gcttcttcca aagtattcag gtatgacccg agacagaact 1141 cctggctgca gatggcagat atgtctgtac cacgctctga atttgctgta ggtgttattg 1201 ggaagtttat ttacgccgta gcaggcagaa ccagagatga gactttctat tcaactgaga 1261 gatatgacat caccaacgat aaatgggaat ttgtggatcc ttatccagtt aacaaatatg 1321 gacatgaggg gacagtgctc aataacaaat tgtttatcac cggtggaatc acctcatctt 1381 ccaccTCCAA GCAAGTGTGC GTGTTTGACC CCAGCAAAGA AGGGACCATA GAACAACGGA 1441 CCAGGAGAAC TCAAGTGGTT ACCAACTGTT GGGAGAATAA GAGCAAGATG AATTACGCGA 1501 GATGCTTTCA CAAGATGATT TCTTACAATG GCAAGCTTTA TGTCTTCGGT GGTGTCTGTG 1561 TGATCTTGAG GGCCTCTTTC GAATCTCAGG GATGCCCTTC TACAGAAGTA TACAACCCAG 1621 AGACTGATCA GTGGACCATC TTGGCATCCA TGCCGATTGG TAGAAGTGGC CATGGTGTGA 1681 CTGTGCTGGA CAAACAAATA ATGGTTCTTG GAGGCCTTTG TTATAATGGT CATTACAGCG 1741 ATTCCATCCT CACTTTTGAT CCGGATGAAA ACAAGTGGAA GGAAGATGAG TACCCTCGGA 1801 TGCCCTGCAA GCTGGATGGT TTACAAGTAT GCAACCTGCA TTTTCCGGAC TATGTACTGG 1861 ATGAGGTCAG GCGTTGCAAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1921 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1981 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGTC CTTGTGGCCT 2041 GCATTCGCGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt