Construct: ORF TRCN0000468830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007273.1_s317c1
- Derived from:
- ccsbBroadEn_13238
- DNA Barcode:
- GTGTAAAAGATTTGCGCTGATTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCBE1 (147372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147372 | CCBE1 | collagen and calcium bindin... | NM_133459.4 | 52.9% | 52.9% | 1_573del |
| 2 | human | 147372 | CCBE1 | collagen and calcium bindin... | XM_024451091.1 | 52.9% | 52.9% | 1_573del |
| 3 | human | 147372 | CCBE1 | collagen and calcium bindin... | XM_017025556.1 | 46.5% | 22.6% | 1_573del;775_887del;1305_1306ins26 |
| 4 | human | 147372 | CCBE1 | collagen and calcium bindin... | XM_017025557.1 | 46.5% | 22.6% | 1_573del;775_887del;1305_1306ins26 |
| 5 | human | 147372 | CCBE1 | collagen and calcium bindin... | XM_017025558.1 | 35.3% | 35% | 1_573del;988_1057del;1098_1099ins190 |
| 6 | human | 147372 | CCBE1 | collagen and calcium bindin... | XR_001753142.1 | 15.7% | (many diffs) | |
| 7 | mouse | 320924 | Ccbe1 | collagen and calcium bindin... | XM_006526021.3 | 55.5% | 57.1% | (many diffs) |
| 8 | mouse | 320924 | Ccbe1 | collagen and calcium bindin... | NM_178793.4 | 46% | 47.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaaagccgga acttgctgtg ccacatgcaa ggagttctac cagatgaagc 121 agaccgtgct gcagctgaag caaaagattg ctctgctccc caacaatgca gctgacctgg 181 gcaagtatat cactggtgac aaggtgctgg cctcaaacac ctaccttcca ggacctcctg 241 gcctgcctgg gggccagggc cctcccggct caccaggacc aaagggaagc ccaggcttcc 301 ccggtatgcc aggcccTCCT GGGCAGCCCG GCCCACGGGG CTCAATGGGA CCCATGGGAC 361 CATCTCCTGA TCTGTCCCAC ATTAAGCAAG GCCGGAGGGG CCCTGTGGGT CCACCAGGGG 421 CACCAGGAAG AGATGGTTCT AAGGGGGAGA GAGGAGCGCC TGGGCCCAGA GGGTCTCCAG 481 GACCCCCTGG TTCTTTCGAC TTCCTGCTAC TTATGCTGGC TGACATCCGC AATGACATCA 541 CTGAGCTGCA GGAAAAGGTG TTCGGGCACC GGACTCACTC TTCAGCAGAG GAGTTCCCTT 601 TACCTCAGGA ATTTCCCAGC TACCCAGAAG CCATGGACCT GGGCTCTGGA GATGACCATC 661 CAAGAAGAAC TGAGACAAGA GACTTGAGAG CCCCCAGAGA CTTCTACCCA TACCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAGTGT AAAAGATTTG CGCTGATTGG ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt