Construct: ORF TRCN0000468848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012473.1_s317c1
- Derived from:
- ccsbBroadEn_01154
- DNA Barcode:
- TGAGTGTGCCCTCCTCGTCATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCP4 (5121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5121 | PCP4 | Purkinje cell protein 4 | NM_006198.3 | 100% | 100% | |
| 2 | mouse | 18546 | Pcp4 | Purkinje cell protein 4 | NM_008791.3 | 89.2% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 119
- ORF end:
- 305
- ORF length:
- 186
- Sequence:
-
1 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 61 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 121 GAGTGAGCGA CAAGGTGCTG GGGCAACCAA TGGAAAAGAC AAGACATCTG GTGAAAATGA 181 TGGACAGAAG AAAGTTCAAG AAGAATTTGA CATTGACATG GATGCACCAG AGACAGAACG 241 TGCAGCGGTG GCCATTCAGT CTCAGTTCAG AAAATTCCAG AAGAAGAAGG CTGGGTCTCA 301 GTCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 361 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 421 TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGAGTGTGCC CTCCTCGTCA TATTACGCGT 481 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt