Construct: ORF TRCN0000468853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002559.1_s317c1
- Derived from:
- ccsbBroadEn_05160
- DNA Barcode:
- GAAACTCGACAAAAGGAAATTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNG3 (170850)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170850 | KCNG3 | potassium voltage-gated cha... | NM_133329.6 | 100% | 100% | |
| 2 | human | 170850 | KCNG3 | potassium voltage-gated cha... | NM_172344.3 | 97.4% | 97.4% | 631_632ins33 |
| 3 | mouse | 225030 | Kcng3 | potassium voltage-gated cha... | NM_153512.1 | 92.7% | 95.8% | (many diffs) |
| 4 | mouse | 225030 | Kcng3 | potassium voltage-gated cha... | XM_006524200.3 | 90.3% | 93.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cttcgggcgc agcggggcgg cctcggtggt gctgaacgtg ggcggcgccc 121 ggtattcgct gtcccgggag ctgctgaagg acttcccgct gcgccgcgtg agccggctgc 181 acggctgccg ctccgagcgc gacgtgctcg aggtgtgcga cgactacgac cgcgagcgca 241 acgagtactt cttcgaccgg cactcggagg ccttcggctt catcctgctc tacgtgcgcg 301 gccacggcaa gctgcgcttc gcgccgcgga tgtgcgagct ctccttctac aacgagatga 361 tctactgggg cctggagggc gcgcacctcg agtactgctg ccagcgccgc ctcgacgacc 421 gcatgtccga cacctacacc ttctactcgg ccgacgagcc gggcgtgctg ggccgcgacg 481 aggcgcgccc cggcggggcc gaggcggctc cctccaggcg ctggctggag cgcatgcggc 541 ggaccttcga ggagcccacg tcgtcgctgg ccgcgcagat cctggctagc gtgtcggtgg 601 tgttcgtgat cgtgtccatg gtggtgctgt gcgccagcac gttgcccgac tggcgcaacg 661 cagccgccga caaccgcagc ctggatgacc ggagcaggta ctccgccggc cctgggaggg 721 agccctccgg gataattgaa gctatctgca taggttggtt cactgccgag tgcatcgtga 781 ggttcattgt ctccaaaaac aagtgtgagt ttgtcaagag acccctgaac atcattgatt 841 tactggcaat cacgccgtat tacatctctg tgttgatgac agtgtttaca ggcgagaact 901 ctcaactcca gagggctgga gtcaccttga gggtacttag aatgatgagg attttttggg 961 tgattaagct tgcccgtcac ttcattggTC TTCAGACACT CGGTTTGACT CTCAAACGTT 1021 GCTACCGAGA GATGGTTATG TTACTTGTCT TCATTTGTGT TGCCATGGCA ATCTTTAGTG 1081 CACTTTCTCA GCTTCTTGAA CATGGGCTGG ACCTGGAAAC ATCCAACAAG GACTTTACCA 1141 GCATTCCTGC TGCCTGCTGG TGGGTGATTA TCTCTATGAC TACAGTTGGC TATGGAGATA 1201 TGTATCCTAT CACAGTGCCT GGAAGAATTC TTGGAGGAGT TTGTGTTGTC AGTGGAATTG 1261 TTCTATTGGC ATTACCTATC ACTTTTATCT ACCATAGCTT TGTGCAGTGT TATCATGAGC 1321 TCAAGTTTAG ATCTGCTAGG TATAGTAGGA GCCTCTCCAC TGAATTCCTG AATTACCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAG AAACTCGACA AAAGGAAATT CATACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att