Construct: ORF TRCN0000468858
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018403.1_s317c1
- Derived from:
- ccsbBroadEn_11966
- DNA Barcode:
- TTAGCCAATTTTATCCTCATCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC174 (51244)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468858
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51244 | CCDC174 | coiled-coil domain containi... | NM_016474.5 | 79.6% | 79.6% | 1_285del |
| 2 | human | 51244 | CCDC174 | coiled-coil domain containi... | XM_017006554.2 | 63.3% | 63.3% | 1_285del;723_724ins228 |
| 3 | human | 51244 | CCDC174 | coiled-coil domain containi... | NR_135523.1 | 34.3% | 1_376del;814_815ins95;1398_2880del | |
| 4 | human | 51244 | CCDC174 | coiled-coil domain containi... | XM_017006555.2 | 31.9% | 31.6% | (many diffs) |
| 5 | mouse | 232236 | Ccdc174 | coiled-coil domain containi... | XM_006505998.3 | 66.9% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac taaaggagac tttatagatg aagaagtaga ggatatgtac cttgtggatt 121 tcacacagaa gatcatagac aagcgcaaag aaatggaggc atctggtgcc catagagatt 181 ctcaaaaggc aggagaaagg gacgacgatg aggaaaacct tcctgaggga gagatccctc 241 ctccccaaga ccccagtgaa gaatgggtgg attacgtgga ctctttgggg cgttcccggc 301 gctgtatgag aaaggatttg ccagatctgc tggagatgga taaaaatctt caggggagac 361 tttttattag tcctgctaat gaaaaaaccc tattatctga agatatgaga aaagaacttc 421 agcgccagca atgggaggaa gaagaaagag aggccctgaa gaggcccatg gggcccgtac 481 attatgaaga cattcgggaa aatgaggccc ggcaacttgg tgttgggtat tttgcctttg 541 cccgagacaa agagttgaga aacaagcaga tgaaaacctt agagatgctg cgtgaacaga 601 caacagatca gagaacaaaa cgagaaaaca taaaggaaaa gcgaaaggct atcttagagg 661 caagacttgc caaacttcga caaaaaaaga tgaaaaaatc aaaagaaggt ggaacagaag 721 aagaaaatag agatggagat gttattgggc ctttgccacc ggagccagag gctgtgccaa 781 ccccacgtcc tgctgcccag agtagcaaag tagaagtcat tgtccaggag aggaaggaca 841 ccaagcctgg agtgccacac atccgggagt gggaccgcgg aaaagaattt tcctttggat 901 actggtcgaa gaggcagtca gatctccggg ctgagagaga tcctgagttt gccccgccgt 961 cagattactt tgtgggtcag aagagaactg gtttttccag cagccaggca tggagcagac 1021 ctgggccagc acagagtgac ccagggcagt gccctgacca gagccacgga cctagccctg 1081 aacatacgtc acccactcct gcccccgaca acccaccaca agcCCCCACA GTTACTTTCA 1141 AAACTCTGGA TGACATGATT TCCTATTACA AACAAGTGAC ATGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGATTA GCCAATTTTA TCCTCATCGA CACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t