Construct: ORF TRCN0000468862
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016732.1_s317c1
- Derived from:
- ccsbBroadEn_06293
- DNA Barcode:
- CTTGGGTGATAGCCACCTCACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAT2 (2780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468862
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2780 | GNAT2 | G protein subunit alpha tra... | NM_005272.4 | 99.9% | 100% | 546G>A |
| 2 | human | 2780 | GNAT2 | G protein subunit alpha tra... | XM_011541264.2 | 99.9% | 100% | 546G>A |
| 3 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | NM_008141.3 | 89.6% | 95.7% | (many diffs) |
| 4 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | XM_006501008.2 | 89.6% | 95.7% | (many diffs) |
| 5 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | XM_006501010.2 | 81.8% | 84.7% | (many diffs) |
| 6 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | XM_006501011.3 | 67% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aagtggagcc agtgctgagg acaaagaact ggccaagagg tccaaggagc 121 tagaaaagaa gctgcaggag gatgctgata aggaagccaa gactgtcaag ctgctactgc 181 tgggtgctgg ggagtcagga aagagcacca tcgtcaaaca gatgaagatc attcaccagg 241 atggctattc accagaagaa tgcctggagt tcaaggctat catctatgga aatgtgctgc 301 agtccatcct ggctatcatc cgggccatga ccacactggg catcgattat gctgaaccaa 361 gctgtgcgga tgacgggcga cagctcaaca acctggctga ctccattgag gagggaacca 421 tgcctcctga gctcgtggag gtcattagga ggttgtggaa ggatggtggg gtgcaagcct 481 gcttcgagag agctgcagaa taccagctta atgactccgc atcttactac ctgaaccaat 541 tagaacgaat tacagaccct gagtacctcc ctagtgagca agatgtgctc cgatccagag 601 tcaaaaccac aggcatcatt gaaaccaagt tttccgtcaa agacttgaaT TTCAGGATGT 661 TTGATGTGGG AGGGCAGAGA TCCGAGAGAA AGAAGTGGAT CCACTGCTTC GAGGGAGTCA 721 CCTGCATCAT TTTCTGTGCA GCCCTCAGTG CCTATGATAT GGTGCTGGTG GAAGATGACG 781 AAGTGAATCG TATGCATGAG TCTTTGCATC TGTTCAACAG CATATGTAAC CACAAATTCT 841 TTGCGGCTAC TTCCATTGTC CTCTTTCTCA ACAAGAAGGA CCTCTTTGAG GAAAAAATCA 901 AGAAAGTCCA TCTCAGCATT TGTTTTCCAG AGTATGATGG TAACAACTCC TATGATGATG 961 CGGGGAATTA CATAAAGAGC CAGTTCCTTG ACCTCAATAT GCGAAAAGAT GTCAAAGAAA 1021 TCTACAGTCA CATGACCTGT GCTACAGATA CACAGAATGT CAAATTTGTG TTTGATGCAG 1081 TTACAGATAT TATCATCAAA GAAAACCTCA AGGACTGCGG CCTCTTCTAC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGACTTGGGT GATAGCCACC TCACTGCACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt