Construct: ORF TRCN0000468863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018445.1_s317c1
- Derived from:
- ccsbBroadEn_15452
- DNA Barcode:
- TGCTTGCAGCATATGAAACTAATT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNRNPF (3185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3185 | HNRNPF | heterogeneous nuclear ribon... | NM_001098204.1 | 99.8% | 99% | 260A>G;1236delT |
| 2 | human | 3185 | HNRNPF | heterogeneous nuclear ribon... | NM_001098205.1 | 99.8% | 99% | 260A>G;1236delT |
| 3 | human | 3185 | HNRNPF | heterogeneous nuclear ribon... | NM_001098206.1 | 99.8% | 99% | 260A>G;1236delT |
| 4 | human | 3185 | HNRNPF | heterogeneous nuclear ribon... | NM_001098207.1 | 99.8% | 99% | 260A>G;1236delT |
| 5 | human | 3185 | HNRNPF | heterogeneous nuclear ribon... | NM_001098208.1 | 99.8% | 99% | 260A>G;1236delT |
| 6 | human | 3185 | HNRNPF | heterogeneous nuclear ribon... | NM_004966.3 | 99.8% | 99% | 260A>G;1236delT |
| 7 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_001166427.1 | 91.4% | 97.1% | (many diffs) |
| 8 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_001166428.1 | 91.4% | 97.1% | (many diffs) |
| 9 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_001166429.1 | 91.4% | 97.1% | (many diffs) |
| 10 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_001166430.1 | 91.4% | 97.1% | (many diffs) |
| 11 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_001166431.1 | 91.4% | 97.1% | (many diffs) |
| 12 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_001166432.1 | 91.4% | 97.1% | (many diffs) |
| 13 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | NM_133834.2 | 91.4% | 97.1% | (many diffs) |
| 14 | mouse | 98758 | Hnrnpf | heterogeneous nuclear ribon... | XM_006506829.3 | 91.4% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1311
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gctgggccct gagggaggtg aaggctttgt ggtcaagctc cgtggcctgc 121 cctggtcctg ctctgttgag gacgtgcaga acttcctctc tgactgcacg attcatgatg 181 gggccgcagg tgtccatttc atctacacta gagagggcag gcagagtggt gaggcttttg 241 ttgaacttgg atcagaagat gatgtaaaaa tggccctgaa aaaagacagg gaaagcatgg 301 gacaccggta cattgaggtg ttcaggtccc acagaaccga gatggattgg gtgttgaagc 361 acagtggtcc caacagtgcc gacagcgcca acgatggctt cgtgcggctt cgaggactcc 421 catttggatg cacaaaggaa gaaattgttc agttcttctc agggttggaa attgtgccaa 481 acgggatcac attgcctgtg gaccccgaag gcaagattac aggggaagcg ttcgtgcagt 541 ttgcctcgca ggagttagct gagaaggctc tagggaaaca caaggagagg atagggcaca 601 ggtacattga ggtgtttaag agcagccagg aggaagttag gtcatactca gatccccctc 661 tgaagttcat gtccgtgcag cggccagggc cctatgaccg gcccgggact gccaggaggt 721 acattggcat cgtgaagcag gcaggcctgg aaaggatgag gcctggtgcc tacagcacag 781 gctacggggg ctacgaggag tacagtggcc tcagtgatgg ctacggcttc accaccgacc 841 tgttcgggag agaccTCAGC TACTGTCTCT CCGGAATGTA TGACCACAGA TACGGCGACA 901 GTGAGTTCAC AGTGCAGAGC ACCACAGGCC ACTGTGTCCA CATGAGGGGC CTGCCGTACA 961 AAGCGACCGA GAACGACATT TACAACTTCT TCTCTCCTCT CAACCCTGTG AGAGTCCATA 1021 TTGAGATTGG CCCAGATGGA AGAGTGACGG GTGAAGCAGA TGTTGAGTTT GCTACTCATG 1081 AAGAAGCTGT GGCAGCTATG TCCAAAGACA GGGCCAATAT GCAGCACAGA TATATAGAAC 1141 TCTTCTTGAA TTCAACAACA GGGGCCAGCA ATGGGGCGTA TAGCAGCCAG GTGATGCAAG 1201 GCATGGGGGT GTCTGCTGCC CAGGCCACTT ACAGTGGCCT GGAGAGCCAG TCAGTGAGTG 1261 GCTGTTACGG GGCCGGCTAC AGTGGGCAGA ACAGCATGGG GGCTATGACT ACCCAACTTT 1321 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 1381 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 1441 TTGTGGAAAG GACGATGCTT GCAGCATATG AAACTAATTA CGCGTTAAGT Cgacaatcaa 1501 cctctggatt acaaaatttg tgaaagatt