Transcript: Mouse NM_001166430.1

Mus musculus heterogeneous nuclear ribonucleoprotein F (Hnrnpf), transcript variant 5, mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Hnrnpf (98758)
Length:
2548
CDS:
667..1914

Additional Resources:

NCBI RefSeq record:
NM_001166430.1
NBCI Gene record:
Hnrnpf (98758)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001166430.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305481 AGCAACGGAGAACGACATTTA pLKO_005 1563 CDS 100% 13.200 18.480 N Hnrnpf n/a
2 TRCN0000109262 GCTCAATTGAGGACGTACAAA pLKO.1 731 CDS 100% 5.625 7.875 N Hnrnpf n/a
3 TRCN0000309854 GCTCAATTGAGGACGTACAAA pLKO_005 731 CDS 100% 5.625 7.875 N Hnrnpf n/a
4 TRCN0000109263 TGCTCAATTGAGGACGTACAA pLKO.1 730 CDS 100% 4.950 6.930 N Hnrnpf n/a
5 TRCN0000305482 AGCATGGGACACCGGTATATT pLKO_005 895 CDS 100% 15.000 12.000 N Hnrnpf n/a
6 TRCN0000109261 GCAGGTGTTCATTTCATTTAT pLKO.1 787 CDS 100% 15.000 10.500 N Hnrnpf n/a
7 TRCN0000351669 GCAGGTGTTCATTTCATTTAT pLKO_005 787 CDS 100% 15.000 10.500 N Hnrnpf n/a
8 TRCN0000305483 AGTATGTTGAGTGGATCTTTA pLKO_005 2192 3UTR 100% 13.200 9.240 N Hnrnpf n/a
9 TRCN0000109260 CCCACTTTGCACCATGAGTTT pLKO.1 2011 3UTR 100% 4.950 3.465 N Hnrnpf n/a
10 TRCN0000109264 CCATTTGGATGCACAAAGGAA pLKO.1 1021 CDS 100% 3.000 2.100 N Hnrnpf n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001166430.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06389 pDONR223 100% 91.4% 97.8% None (many diffs) n/a
2 ccsbBroad304_06389 pLX_304 0% 91.4% 97.8% V5 (many diffs) n/a
3 TRCN0000467459 AGTGAAGATAGCCTCTACCCTTTA pLX_317 36% 91.4% 97.8% V5 (many diffs) n/a
4 ccsbBroadEn_06388 pDONR223 100% 91.4% 98% None (many diffs) n/a
5 ccsbBroad304_06388 pLX_304 0% 91.4% 98% V5 (many diffs) n/a
6 TRCN0000468107 TCTATCCATATGGATCACAATGTT pLX_317 36.8% 91.4% 98% V5 (many diffs) n/a
7 ccsbBroadEn_15452 pDONR223 0% 91.4% 97.8% None (many diffs) n/a
8 ccsbBroad304_15452 pLX_304 0% 91.4% 97.8% V5 (many diffs) n/a
9 TRCN0000468863 TGCTTGCAGCATATGAAACTAATT pLX_317 36.7% 91.4% 97.1% V5 (not translated due to frame shift) (many diffs) n/a
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