Construct: ORF TRCN0000468865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000970.1_s317c1
- Derived from:
- ccsbBroadEn_13907
- DNA Barcode:
- TAATTGATAGTTTATCCTCCCACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYL3 (4634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4634 | MYL3 | myosin light chain 3 | NM_000258.3 | 99.6% | 2% | 9_9delCinsGN |
| 2 | mouse | 17897 | Myl3 | myosin, light polypeptide 3 | NM_010859.2 | 86.9% | 1.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 99
- ORF length:
- 33
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccgnaaaaa gccagagccc aagaaggatg atgccaaggc agcccccaag 121 gcagctccag ctcccgcacc tccccctgag cctgagcgcc ctaaggaggt cgagtttgat 181 gcttccaaga tcaagattga gttcacaccT GAGCAGATTG AAGAGTTCAA GGAAGCCTTC 241 ATGCTGTTCG ACCGCACACC CAAGTGTGAG ATGAAGATCA CCTACGGGCA GTGTGGGGAT 301 GTCCTGCGGG CGCTGGGCCA GAACCCCACA CAGGCAGAAG TGCTCCGTGT CCTGGGGAAG 361 CCAAGACAGG AAGAGCTCAA TACCAAGATG ATGGACTTTG AAACTTTCCT GCCTATGCTC 421 CAGCACATTT CCAAGAACAA GGACACAGGC ACCTATGAGG ACTTCGTGGA GGGGCTGCGG 481 GTCTTCGACA AGGAGGGCAA TGGCACTGTC ATGGGTGCTG AGCTTCGCCA CGTGCTGGCC 541 ACGCTGGGTG AGAGGCTGAC AGAAGACGAA GTGGAGAAGT TGATGGCTGG GCAAGAGGAC 601 TCCAATGGCT GCATCAACTA TGAAGCATTT GTGAAGCACA TCATGTCCAG CTACCCAACT 661 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 721 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 781 TCTTGTGGAA AGGACGATAA TTGATAGTTT ATCCTCCCAC CACGCGTTAA GTCgacaatc 841 aacctctgga ttacaaaatt tgtgaaagat t