Construct: ORF TRCN0000468878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001791.1_s317c1
- Derived from:
- ccsbBroadEn_00612
- DNA Barcode:
- AAGAAAGTACTACATAAAATAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNS (2588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | NM_000512.5 | 100% | 100% | |
| 2 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | NM_001323544.2 | 93.7% | 90.2% | (many diffs) |
| 3 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | XM_017023111.2 | 90.2% | 84.7% | (many diffs) |
| 4 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | XM_017023112.2 | 89.5% | 85.2% | (many diffs) |
| 5 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | XM_005256301.3 | 89.4% | 86.1% | (many diffs) |
| 6 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | XM_011522982.2 | 84% | 77.7% | (many diffs) |
| 7 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | NM_001323543.2 | 64.5% | 64.5% | 0_1ins555 |
| 8 | human | 2588 | GALNS | galactosamine (N-acetyl)-6-... | XM_017023113.1 | 57.3% | 54.3% | (many diffs) |
| 9 | mouse | 50917 | Galns | galactosamine (N-acetyl)-6-... | NM_016722.4 | 83.5% | 85% | (many diffs) |
| 10 | mouse | 50917 | Galns | galactosamine (N-acetyl)-6-... | NM_001193645.1 | 70.6% | 72.4% | (many diffs) |
| 11 | mouse | 50917 | Galns | galactosamine (N-acetyl)-6-... | XR_387755.1 | 46.5% | (many diffs) | |
| 12 | mouse | 50917 | Galns | galactosamine (N-acetyl)-6-... | XR_387754.1 | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1632
- ORF length:
- 1566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggttgtc gcggcgacga ggtggtggca gctgttgctg gtgctcagcg 121 ccgcggggat gggggcctcg ggcgccccgc agccccccaa catcctgctc ctgctcatgg 181 acgacatggg atggggtgac ctcggggtgt atggagagcc ctccagagag accccgaatt 241 tggaccggat ggctgcagaa gggctgcttt tcccaaactt ctattctgcc aaccctctgt 301 gctcgccatc gagggcggca ctgctcacag gacggctacc catccgcaat ggcttctaca 361 ccaccaacgc ccatgccaga aacgcctaca caccgcagga gattgtgggc ggcatcccag 421 actcggagca gctcctgccg gagcttctga agaaggccgg ctacgtcagc aagattgtcg 481 gcaagtggca tctgggtcac aggccccagt tccaccccct gaagcacgga tttgatgagt 541 ggtttggatc ccccaactgc cactttggac cttatgacaa caaggccagg cccaacatcc 601 ctgtgtacag ggactgggag atggttggca gatattatga agaatttcct attaatctga 661 agacggggga agccaacctc acccagatct acctgcagga agccctggac ttcattaaga 721 gacaggcacg gcaccacccc tttttcctct actgggctgt cgacgccacg cacgcacccg 781 tctatgcctc caaacccttc ttgggcacca gtcagcgagg gcggtatgga gacgccgtcc 841 gggagattga tgacagcatt gggaagatac tggagctcct ccaagacctg cacgtcgcgg 901 acaacacctt cgtcttcttc acgtcggaca acggcgctgc cctcatttcc gcccccgaac 961 aaggtggcag caacggcccc tttctgtgtg ggaagcagac cacgtttgaa ggagggatga 1021 gggagcctgc cctcgcatgg tggccagggc acgtcactgc aggccaggtg agccaccagc 1081 tgggcagcat catggacctc ttcaccacca gcctggccct tgcgggcctg acgccgccca 1141 gcgacagggc cattgatggc ctcaacctcc tccccacccT CCTGCAGGGC CGGCTGATGG 1201 ACAGGCCTAT CTTCTATTAC CGTGGCGACA CGCTGATGGC GGCCACCCTC GGGCAGCACA 1261 AGGCTCACTT CTGGACCTGG ACCAACTCCT GGGAGAACTT CAGACAGGGC ATTGATTTCT 1321 GCCCTGGGCA GAACGTTTCA GGGGTCACAA CTCACAATCT GGAAGACCAC ACGAAGCTGC 1381 CCCTGATCTT CCACCTGGGA CGGGACCCAG GGGAGAGGTT CCCCCTCAGC TTTGCCAGCG 1441 CCGAGTACCA GGAGGCCCTC AGCAGGATCA CCTCGGTCGT CCAGCAGCAC CAGGAGGCCT 1501 TGGTCCCCGC GCAGCCCCAG CTCAACGTGT GCAACTGGGC GGTCATGAAC TGGGCACCTC 1561 CGGGCTGTGA AAAGTTAGGG AAGTGTCTGA CACCTCCAGA ATCCATTCCC AAGAAGTGCC 1621 TCTGGTCCCA CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1741 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAG AAAGTACTAC ATAAAATAAC 1801 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t