Construct: ORF TRCN0000468892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017439.1_s317c1
- Derived from:
- ccsbBroadEn_07946
- DNA Barcode:
- TCGAACATGGCGCCTAAATATTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMM50 (25813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25813 | SAMM50 | SAMM50 sorting and assembly... | NM_015380.5 | 99.6% | 99.5% | (many diffs) |
| 2 | mouse | 68653 | Samm50 | SAMM50 sorting and assembly... | NM_178614.4 | 85% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gactgtgcac gcccggagtt tggagcctct tccatcaagt ggacctgatt 121 ttggaggatt aggagaagaa gctgaatttg ttgaagttga gcctgaagct aaacaggaaa 181 ttcttgaaaa caaagatgtg gttgttcaac atgttcattt tgatggactt ggaaggacta 241 aagataatat catcatttgt gaaattggag atgttttcaa ggccaaaaac ctaattgagg 301 taatgcggaa atctcatgaa gcccgtgaaa aattgctccg tcttggaatt tttagacaag 361 tggatgtttt gattgacaca tgtcaaggtg atgacgcact tccaaatggg ttagacgtta 421 cctttgaagt aactgaattg aggagattaa cgggcagtta taacaccatg gttgggaaca 481 atgaaggcag tatggtactt ggcctcaagc ttcctaatct tcttggtcgt gcagaaaagg 541 tgacctttca gttttcctat ggaacaaaag aaacttcgta tggcctgtcc ttcttcaaac 601 cacggcccgg aaacttcgaa agaaatttct ctgtaaactt atataaagtt actggacagt 661 tcccttggag ctcactgcgg gagacggaca gaggaatgtc agctgagtac agttttccca 721 tatggaagac cagccacact gtcaagtggg aaggcgtatg gcgagaactg ggctgcctct 781 caaggacggc gtcatttgct gttcgaaaag aaagcggaca ttcactgaaa tcatctcttt 841 cgcacgccat ggtcatcgat tctcggaatt cttccatctt accaaggaga ggtgctttgc 901 tgaaagttaa ccaggaactg gcaggctaca ctggcgggga tgtgagcttc atcaaagaag 961 attttgaact tcagttgaac aagcaactca tatttgattc agttttttca gcgtctttct 1021 gggGCGGAAT GTTGGTACCC ATTGGTGATA AGCCGTCAAG CATTGCTGAT AGGTTTTACC 1081 TCGGGGGACC CACAAGCGTC CGCGGATTCA GCATGCACAG CATCGGGCCA CAGAGCGAAG 1141 GAGACTACCT AGGTGGAGAA GCGTACTGGG CCGGCGGCCT GCACCTCTAC ACCCCATTAC 1201 CTTTCCGGCC AGGCCAGGGT GGCTTTGGAG AACTTTTCCG AACACACTTC TTTCTCAACG 1261 CAGGAAACCT CTGCAACCTC AACTATGGGG AGGGCCCCAA AGCTCATATT CGTAAGCTGG 1321 CTGAGTGCAT CCGCTGGTCG TACGGGGCCG GGATTGTCCT CAGGCTTGGC AACATCGCTC 1381 GGTTGGAACT TAATTACTGC GTCCCCATGG GAGTACAGAC AGGTGACAGG ATATGTGATG 1441 GCGTCCAGTT TGGAGCTGGG ATAAGGTTCC TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1621 GAACATGGCG CCTAAATATT AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt