Construct: ORF TRCN0000468897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004110.3_s317c1
- Derived from:
- ccsbBroadEn_15327
- DNA Barcode:
- TAATAACTGCTATCGATAGTCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK4 (440275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468897
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440275 | EIF2AK4 | eukaryotic translation init... | NM_001013703.4 | 48.8% | 48.8% | 1_2529del;3916G>T |
| 2 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_017318915.1 | 48.8% | 51.6% | (many diffs) |
| 3 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_006499627.3 | 47.5% | 50.2% | (many diffs) |
| 4 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_006499626.3 | 46.7% | 49.3% | (many diffs) |
| 5 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | NM_001177806.1 | 46.4% | 49% | (many diffs) |
| 6 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_006499625.3 | 46.4% | 49% | (many diffs) |
| 7 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_017318909.1 | 43.9% | 46.4% | (many diffs) |
| 8 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | NM_013719.3 | 43.2% | 45.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2484
- ORF length:
- 2418
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tcaccgggat ttgaagcctg tcaacatttt tttggattct gatgaccatg 121 tgaaaatagg tgattttggt ttggcgacag accatctagc cttttctgct gacagcaaac 181 aagacgatca gacaggagac ttgattaagt cagacccttc aggtcactta actgggatgg 241 ttggcactgc tctctatgta agcccagagg tccaaggaag caccaaatct gcatacaacc 301 agaaagtgga tctcttcagc ctgggaatta tcttctttga gatgtcctat caccccatgg 361 tcacggcttc agaaaggatc tttgttctca accaactcag agatcccact tcgcctaagt 421 ttccagaaga ctttgacgat ggagagcatg caaagcagaa atcagtcatc tcctggctgt 481 tgaaccacga tccagcaaaa cggcccacag ccacagaact gctcaagagt gagctgctgc 541 ccccacccca gatggaggag tcagagctgc atgaagtgct gcaccacacg ctgaccaacg 601 tggatgggaa ggcctaccgc accatgatgg cccagatctt ctcgcagcgc atctcccctg 661 ccatcgatta cacctatgac agcgacatac tgaagggcaa cttctcaatc cgtacagcca 721 agatgcagca gcatgtgtgt gaaaccatca tccgcatctt taaaagacat ggagctgttc 781 agttgtgtac tccactactg cttccccgaa acagacaaat atatgagcac aacgaagctg 841 ccctattcat ggaccacagc gggatgctgg tgatgcttcc ttttgacctg cggatccctt 901 ttgcaagata tgtggcaaga aataatatat tgaatttaaa acgatactgc atagaacgtg 961 tgttcaggcc gcgcaagtta gatcgatttc atcccaaaga acttctggag tgtgcatttg 1021 atattgtcac ttctaccacc aacagctttc tgcccactgc tgaaattatc tacactatct 1081 atgaaatcat ccaagagttt ccagcacttc aggaaagaaa ttacagtatt tatttgaacc 1141 ataccatgtt attgaaagca atactcttac actgtgggat cccagaagat aaactcagtc 1201 aagtctacat tattctgtat gatgctgtga cagagaagct gacgaggaga gaagtggaag 1261 ctaaattttg taatctgtct ttgtcttcta atagtctgtg tcgactctac aagtttattg 1321 aacagaaggg agatttgcaa gatcttatgc caacaataaa ttcattaata aaacagaaaa 1381 caggtattgc acagttggtg aagtatggct taaaagacct agaggaggtt gttggactgt 1441 tgaagaaact ctgcatcaag ttacaggtct tgatcaattt gggcttggtt tacaaggtgc 1501 agcagcacaa tggaatcatc ttccagtttg tggctttcat caaacgaagg caaagggctg 1561 tacctgaaat cctcgcagct ggaggcagat atgacctgct gattccccag tttagagggc 1621 cacaagctct ggggccagtt cccactgcca ttggggtcag catagctata gacaagatat 1681 ctgctgctgt cctcaacatg gaggaatctg ttacaataag ctcttgtgac ctcctggttg 1741 taagtgttgg ccagatgtct atgtccaggg ccatcaacct aacccagaaa ctctggacag 1801 caggcatcac agcagaaatc atgtacgact ggtcacagtc ccaagaggaa ttacaagagt 1861 actgcagaca tcatgaaatc acctatgtgg cccttgtctc ggataaagaa ggaagccatg 1921 tcaaggttaa gtctttcgag aaggaaaggc agacagagaa gcgtgtgctg gagactgaac 1981 ttgtggacca tgtactgcag aaactgagga ctaaagtcac tgatgaaagg aatggcagag 2041 aagcttccga taatcttgca gtgcaaaatc tgaaggggtc attttctaat gcttcaggtt 2101 tGTTTGAAAT CCATGGAGCA ACAGTGGTTC CCATTGTGAG TGTGCTAGCC CCGGAGAAGC 2161 TGTCAGCCAG CACTAGGAGG CGCTATGAAA CTCAGGTACA AACTCGACTT CAGACCTCCC 2221 TTGCCAACTT ACATCAGAAA AGCAGTGAAA TTGAAATTCT GGCTGTGGAT CTACCCAAAG 2281 AAACAATATT ACAGTTTTTA TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA 2341 CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAAAGATA CCTCAAATTA GTCTGTGATG 2401 AAATTTATAA CATCAAAGTA GAAAAAAAGG TGTCTGTGCT ATTTCTGTAC AGCTATAGAG 2461 ATGACTACTA CAGAATCTTA TTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2521 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2581 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATAACTGCT 2641 ATCGATAGTC CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2701 att