Construct: ORF TRCN0000468900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007288.2_s317c1
- Derived from:
- ccsbBroadEn_14710
- DNA Barcode:
- GCGGAGTCGCTCCTGGGTCCTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEK2 (4751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4751 | NEK2 | NIMA related kinase 2 | NM_001204183.1 | 55.6% | 55.2% | 504T>C;639_652del;657_1152del |
| 2 | human | 4751 | NEK2 | NIMA related kinase 2 | NM_001204182.1 | 55% | 54.6% | 504T>C;639_652del;657_1164del |
| 3 | human | 4751 | NEK2 | NIMA related kinase 2 | XM_005273147.2 | 48.8% | 48.5% | 504T>C;639_652del;657_1311del |
| 4 | human | 4751 | NEK2 | NIMA related kinase 2 | NM_002497.4 | 48% | 47.6% | 504T>C;639_652del;657_1335del |
| 5 | mouse | 18005 | Nek2 | NIMA (never in mitosis gene... | XM_006497123.3 | 42.2% | 45.6% | (many diffs) |
| 6 | mouse | 18005 | Nek2 | NIMA (never in mitosis gene... | NM_010892.3 | 41.5% | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ttcccgggct gaggactatg aagtgttgta caccattggc acaggctcct 121 acggccgctg ccagaagatc cggaggaaga gtgatggcaa gatattagtt tggaaagaac 181 ttgactatgg ctccatgaca gaagctgaga aacagatgct tgtttctgaa gtgaatttgc 241 ttcgtgaact gaaacatcca aacatcgttc gttactatga tcggattatt gaccggacca 301 atacaacact gtacattgta atggaatatt gtgaaggagg gGATCTGGCT AGTGTAATTA 361 CAAAGGGAAC CAAGGAAAGG CAATACTTAG ATGAAGAGTT TGTTCTTCGA GTGATGACTC 421 AGTTGACTCT GGCCCTGAAG GAATGCCACA GACGAAGTGA TGGTGGTCAT ACCGTATTGC 481 ATCGGGATCT GAAACCAGCC AATGTTTTCC TGGATGGCAA GCAAAACGTC AAGCTTGGAG 541 ACTTTGGGCT AGCTAGAATA TTAAACCACG ACACGAGTTT TGCAAAAACA TTTGTTGGCA 601 CACCTTATTA CATGTCTCCT GAACAAATGA ATCGCATGTC CTACAATGAG AAATCAGATA 661 TCTGGTCATT GGGCTGCTTG CTGTATGAGT TATGTGCATT AATCTTTTAC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGAGCGGAGT CGCTCCTGGG TCCTTAGACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt