Construct: ORF TRCN0000468903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018890.3_s317c1
- Derived from:
- ccsbBroadEn_14974
- DNA Barcode:
- ATTTGGCAGTTAAACTTCTAGGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K2 (10746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468903
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10746 | MAP3K2 | mitogen-activated protein k... | NM_001371910.2 | 98.6% | 34.5% | (many diffs) |
| 2 | human | 10746 | MAP3K2 | mitogen-activated protein k... | NM_001371911.1 | 98.6% | 34.5% | (many diffs) |
| 3 | human | 10746 | MAP3K2 | mitogen-activated protein k... | NM_006609.5 | 98.6% | 34.5% | (many diffs) |
| 4 | mouse | 26405 | Map3k2 | mitogen-activated protein k... | NM_011946.3 | 90.7% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 828
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgatcag caagctttga actcaatcat gcaagatttg gctgtccttc 121 ataaggccag tcgaccagca ttatccttgc aggaaaccag aaaagcaaaa tcttcatcac 181 caaaaaaaca gaatgatgtc cgagtcaaat ttgaacatag aggagaaaaa agaatccttc 241 agttccccag accagttaaa ctggaagatc tgagatctaa agctaaaatt gcctttggac 301 agtctatgga tctacattat accaataacg agttggtaat tccattaact actcaagatg 361 acttggacaa agctgtggaa ctgctggatc gtagtattca tatgaagagc ctcaagatat 421 tacttgtaat aaatggaagt acacaggcta ctaatttaga accattgcca tcactagaag 481 atttggataa tacagtattt ggagcagaga ggaaaaaacg gctatctata ataggtccta 541 ctagtagaga tagaagttct cctcccccag gttacattcc agatgaatta caccaggttg 601 cccggaatgg gtcattcact agtatcaaca gtgaaggaga gttcattcca gagagcatgg 661 accaaatgct ggatccatta tctttaagca gccctgaaaa ttctggctca gaagttgtcc 721 atcacttgat agtcctttgg atggagagag ctatccaaaa tcacgaatgc ctagggctca 781 gagctaccca gatatcatca gattttcaga ctatgatacc ctatctttga gaattgggaa 841 agagacatat ccagaaggta tcatgtttca tatcatcatc aagagtataa tgatggtcgt 901 aaaacttttc caagagctag aaggacccag gggaccagct tacggtctcc tgtgagtttc 961 agtcctactg atcattcctt aagcactagt agtggaagca gtatctttac cccagagtat 1021 gatgatagtc gaataagaag aaggggaagt gacatagaca atcctacttt gaccgtaatg 1081 gacatcagcc cacccagccg ttcacctcga gctccgacca cttggaagat gggcaaactg 1141 cttgcccaag gagcctttgg naaggtctac ctctgttatg atgtgattac aggaagagaa 1201 tttgctgtaa gcaaagttca atttgacccc gatagtcntg agaccagcaa ggaagtaaat 1261 gcacttgagt gtgaaattca gttgctgaaa aacttgctac atgagcgaat tgttcagtat 1321 tatggctgtt tgagggatcc ccaggaaaaa acactttcca tatttatgga atatatgcca 1381 gggggttcaa ttaaggacca attaaaagca tatggcgctc ttactgagaa tgtgactagg 1441 aaatacaccc gtcagattct ggagggtgtc cattatttgc acagtaatat gattgtccat 1501 agagatatca aaggcgcaaa tatcctgcga gattcaacag gcaacgtcaa actaggagat 1561 tttGGGGCCA GCAAACGGCT TCAGACCATC TGTCTCTCAG GGACAGGAAT GAAGTCTGTC 1621 ACGGGCACAC CATACTGGAT GAGCCCTGAA GTCATCAGTG GAGAAGGCTA TGGAAGAAAA 1681 GCAGACATCT GGAGTGTTGC ATGTACTGTG GTAGAAATGC TAACTGAAAA GCCGCCTTGG 1741 GCTGAATTTG AAGCAATGGC TGCCATCTTT AAAATCGCCA CTCAGCCAAC AAACCCAAAG 1801 CTGCCACCTC ATGTCTCAGA CTATACTCGA GATTTCCTCA AACGGATTTT TGTAGAGGCC 1861 AAACTGAGAC CTTCAGCTGA TGAACTCTTA AGGCACATGT TTGTGCATTA TCACTTGCCA 1921 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 1981 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 2041 ATATCTTGTG GAAAGGACGA ATTTGGCAGT TAAACTTCTA GGGGACGCGT TAAGTCgaca 2101 atcaacctct ggattacaaa atttgtgaaa gatt