Construct: ORF TRCN0000468904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018888.3_s317c1
- Derived from:
- ccsbBroadEn_14812
- DNA Barcode:
- AGTTTCAGCGAAGGTGATAATAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EIF2AK2 (5610)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135651.3 | 96.3% | 43.1% | (many diffs) |
| 2 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_002759.3 | 96.3% | 43.1% | (many diffs) |
| 3 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_011532987.2 | 96.3% | 43.1% | (many diffs) |
| 4 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135652.2 | 89.1% | 46.5% | 715_717delGCAinsC;785_786ins123;1357_1410del |
| 5 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_017004503.1 | 88.5% | 46.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 807
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctggtgat ctttcagcag gtttcttcat ggaggaactt aatacatacc 121 gtcagaagca gggagtagta cttaaatatc aagaactgcc taattcagga cctccacatg 181 ataggaggtt tacatttcaa gttataatag atggaagaga atttccagaa ggtgaaggta 241 gatcaaagaa ggaagcaaaa aatgccgcag ccaaattagc tgttgagata cttaataagg 301 aaaagaaggc agttagtcct ttattattga caacaacgaa ttcttcagaa ggattatcca 361 tggggaatta cataggcctt atcaatagaa ttgcccagaa gaaaagacta actgtaaatt 421 atgaacagtg tgcatcgggg gtgcatgggc cagaaggatt tcattataaa tgcaaaatgg 481 gacagaaaga atatagtatt ggtacaggtt ctactaaaca ggaagcaaaa caattggccg 541 ctaaacttgc atatcttcag atattatcag aagaaacctc agtgaaatct gactacctgt 601 cctctggttc ttttgctact acgtgtgagt cccaaagcaa ctctttagtg accagcacac 661 tcgcttctga atcatcatct gaaggtgact tctcagcaga tacatcagag ataaattcta 721 acagtgacag tttaaacagt tcttcgttgc ttatgaatgg tctcagaaat aatcaaagga 781 agcaaaagat ctttggcacc cagatttgac cttcctgaca tgaaagaaac aaagtatact 841 gtggacaaga ggtttggcat ggattttaaa gaaatagaat taattggctc aggtggattt 901 ggccaagttt tcaaagcaaa acacagaatt gaccggaaga cttacggtat taaacgtgtt 961 aaatataata acgagaaggc ggagcgtgaa gtaaaagcat tggcaaaact tgatcatgta 1021 aatattgttc actacaatgg ctgttgggat ggatttgatt atgatcctga gaccagtgat 1081 gattctcttg agagcagtga ttatgatcct gagaacagca aaaatagttc aaggtcaaag 1141 actaagtgcc ttttcatcca aatggaattc tgtgataaag ggaccttgga acaatggatt 1201 gaaaaaagaa gaggcgagaa actagacaaa gttttggctt tggaactctt tgaacaaata 1261 acaaaagggg tggattatat acattcaaaa aaattaattc atagagaTCT TAAGCCAAGT 1321 AATATATTCT TAGTAGATAC AAAACAAGTA AAGATTGGAG ACTTTGGACT TGTAACATCT 1381 CTGAAAAATG ATGGAAAGCG AACAAGGAGT AAGGGAACTT TGCGATACAT GAGCCCAGAA 1441 CAGATTTCTT CGCAAGACTA TGGAAAGGAA GTGGACCTCT ACGCTTTGGG GCTAATTCTT 1501 GCTGAACTTC TTCATGTATG TGACACTGCT TTTGAAACAT CAAAGAAAAC TCTTCTACAG 1561 AAATTACTCT CAAAGAAACC TGAGGATCGA CCTAACACAT CTGAAATACT AAGGACCTTG 1621 ACTGTGTGGA AGAAAAGCCC AGAGAAAAAT GAACGACACA CATGTTTGCC AACTTTCTTG 1681 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 1741 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 1801 GGAAAGGACG AAGTTTCAGC GAAGGTGATA ATAGAACGCG TTAAGTCgac aatcaacctc 1861 tggattacaa aatttgtgaa agatt