Construct: ORF TRCN0000468908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016174.1_s317c1
- Derived from:
- ccsbBroadEn_04360
- DNA Barcode:
- TCCGCGGTTCAGGCTATTGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPNS2 (84290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84290 | CAPNS2 | calpain small subunit 2 | NM_032330.3 | 100% | 100% | |
| 2 | mouse | 69543 | Capns2 | calpain, small subunit 2 | NM_027112.1 | 85.9% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcttgcaaag gctctattgg aaggagcaga tcgaggtctt ggagaagctc 121 ttggaggcct ctttggagga ggtggtcaga gaagagaagg aggaggaaga aatattggag 181 ggatagttgg aggaattgtg aattttatca gtgaggctgc agcagctcag tatactccag 241 aaccgcctcc cactcagcag catttcacca gtgtggaggc ctcagaaagt gaggaagtta 301 ggcgatttcg gcaacaattt acacagctgg ctggaccaga catggaggtg ggtgccactg 361 atcTGATGAA TATTCTCAAC AAAGTCCTTT CTAAGCACAA AGATCTTAAG ACTGACGGTT 421 TTAGTCTTGA CACCTGCCGG AGCATTGTGT CTGTCATGGA CAGTGACACG ACTGGTAAGC 481 TGGGCTTTGA AGAATTTAAG TATCTGTGGA ACAACATCAA GAAATGGCAG TGTGTTTATA 541 AGCAGTATGA CAGGGACCAT TCTGGGTCTC TGGGAAGTTC TCAGCTGCGG GGAGCTCTGC 601 AGGCCGCAGG CTTCCAGCTA AATGAACAAC TTTACCAAAT GATTGTCCGC CGGTATGCTA 661 ATGAAGATGG AGATATGGAT TTTAACAATT TCATCAGCTG CTTGGTCCGC CTGGATGCCA 721 TGTTTCGTGC CTTCAAGTCT CTGGATAGAG ATAGAGATGG CCTGATTCAA GTGTCTATCA 781 AAGAATGGCT GCAGTTGACC ATGTATTCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 841 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 901 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATT TTGTGGAAAG GACGATCCGC 961 GGTTCAGGCT ATTGAACCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1021 tgaaagatt