Construct: ORF TRCN0000468924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006213.1_s317c1
- Derived from:
- ccsbBroadEn_01087
- DNA Barcode:
- AAGGCCGCCAGGCTCCATGAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NF2 (4771)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4771 | NF2 | neurofibromin 2 | NM_181830.3 | 100% | 100% | |
| 2 | human | 4771 | NF2 | neurofibromin 2 | NM_181831.3 | 100% | 100% | |
| 3 | human | 4771 | NF2 | neurofibromin 2 | NM_181828.3 | 92.5% | 92.5% | 113_235del |
| 4 | human | 4771 | NF2 | neurofibromin 2 | NM_181829.3 | 92.3% | 92.3% | 113_238del |
| 5 | human | 4771 | NF2 | neurofibromin 2 | XM_017028810.1 | 88.6% | 86.4% | (many diffs) |
| 6 | human | 4771 | NF2 | neurofibromin 2 | XM_017028809.2 | 86.6% | 83.8% | (many diffs) |
| 7 | human | 4771 | NF2 | neurofibromin 2 | NM_016418.5 | 85.9% | 85.9% | 113_361del |
| 8 | human | 4771 | NF2 | neurofibromin 2 | NM_181825.3 | 85.9% | 85.9% | 113_361del |
| 9 | human | 4771 | NF2 | neurofibromin 2 | NM_181832.3 | 85.9% | 85.9% | 113_361del |
| 10 | human | 4771 | NF2 | neurofibromin 2 | NM_000268.3 | 84% | 83.5% | (many diffs) |
| 11 | human | 4771 | NF2 | neurofibromin 2 | NR_156186.2 | 24.9% | (many diffs) | |
| 12 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_006514573.2 | 83.1% | 90.5% | (many diffs) |
| 13 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_006514571.3 | 81.4% | 86.9% | (many diffs) |
| 14 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_017314334.1 | 81.1% | 87.9% | (many diffs) |
| 15 | mouse | 18016 | Nf2 | neurofibromin 2 | NM_001252253.1 | 78.5% | 82.6% | (many diffs) |
| 16 | mouse | 18016 | Nf2 | neurofibromin 2 | NM_001252250.1 | 77.3% | 84.2% | (many diffs) |
| 17 | mouse | 18016 | Nf2 | neurofibromin 2 | NM_001252251.1 | 77.3% | 84.2% | (many diffs) |
| 18 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_006514570.2 | 77.3% | 84.2% | (many diffs) |
| 19 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_011243669.2 | 77.3% | 84.2% | (many diffs) |
| 20 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_006514569.3 | 75.8% | 81% | (many diffs) |
| 21 | mouse | 18016 | Nf2 | neurofibromin 2 | NM_001252252.1 | 75.6% | 82.4% | (many diffs) |
| 22 | mouse | 18016 | Nf2 | neurofibromin 2 | NM_010898.4 | 75.6% | 81.8% | (many diffs) |
| 23 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_006514574.3 | 73.8% | 78.9% | (many diffs) |
| 24 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_011243670.2 | 71.5% | 75.9% | (many diffs) |
| 25 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_017314335.1 | 70.2% | 76.6% | (many diffs) |
| 26 | mouse | 18016 | Nf2 | neurofibromin 2 | XM_006514572.3 | 68.8% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1587
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggggccatc gcttcccgca tgagcttcag ctctctcaag aggaagcaac 121 ccaagacgtt caccgtgagg atcgtcacca tggacgccga gatggagttc aattgcgagg 181 taaagaagca gattttagat gaaaagatct actgccctcc tgaggcttct gtgctcctgg 241 cttcttacgc cgtccaggcc aagtatggtg actacgaccc cagtgttcac aagcggggat 301 ttttggccca agaggaattg cttccaaaaa gggtaataaa tctgtatcag atgactccgg 361 aaatgtggga ggagagaatt actgcttggt acgcagagca ccgaggccga gccagggatg 421 aagctgaaat ggaatatctg aagatagctc aggacctgga gatgtacggt gtgaactact 481 ttgcaatccg gaataaaaag ggcacagagc tgctgcttgg agtggatgcc ctggggcttc 541 acatttatga ccctgagaac agactgaccc ccaagatctc cttcccgtgg aatgaaatcc 601 gaaacatctc gtacagtgac aaggagttta ctattaaacc actggataag aaaattgatg 661 tcttcaagtt taactcctca aagcttcgtg ttaataagct gattctccag ctatgtatcg 721 ggaaccatga tctatttatg aggagaagga aagccgattc tttggaagtt cagcagatga 781 aagcccaggc cagggaggag aaggctagaa agcagatgga gcggcagcgc ctcgctcgag 841 agaagcagat gagggaggag gctgaacgca cgagggatga gttggagagg aggctgctgc 901 agatgaaaga agaagcaaca atggccaacg aagcactgat gcggtctgag gagacagctg 961 acctgttggc tgaaaaggcc cagatcaccg aggaggaggc aaaacttctg gcccagaagg 1021 ccgcagaggc tgagcaggaa atgcagcgca tcaaggccac agcgattcgc acggaggagg 1081 agaagcgcct gatggagcag aaggtgctgg aagccgaggt gctggcactg aagatggctg 1141 aggagtcaga gaggagggcc aaagaggcag aTCAGCTGAA GCAGGACCTG CAGGAAGCAC 1201 GCGAGGCGGA GCGAAGAGCC AAGCAGAAGC TCCTGGAGAT TGCCACCAAG CCCACGTACC 1261 CGCCCATGAA CCCAATTCCA GCACCGTTGC CTCCTGACAT ACCAAGCTTC AACCTCATTG 1321 GTGACAGCCT GTCTTTCGAC TTCAAAGATA CTGACATGAA GCGGCTTTCC ATGGAGATAG 1381 AGAAAGAAAA AGTGGAATAC ATGGAAAAGA GCAAGCATCT GCAGGAGCAG CTCAATGAAC 1441 TCAAGACAGA AATCGAGGCC TTGAAACTGA AAGAGAGGGA GACAGCTCTG GATATTCTGC 1501 ACAATGAGAA CTCCGACAGG GGTGGCAGCA GCAAGCACAA TACCATTAAA AAGCCTCAAG 1561 CCCAAGGCAG AAGACCTATC TGCATTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGGCCGC 1741 CAGGCTCCAT GAATCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt